Warm cells revealed by microfluorimetry of Ca in cultured dorsal root ganglion neurons |
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Authors: | H Gotoh H Akatsuka K Suto |
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Abstract: | Warm cells were identified by Fura-PE3-based microfluorimetry of Ca2+ in cultured dorsal root ganglion (DRG) neurons. In response to a physiologically relevant stimulus temperature (43°C), a subpopulation of small DRG neurons from new born rats increased the intracellular Ca2+ concentration (Ca2+]i). Seven percent of the cells responded to the warm stimulus. The stimulus evoked elevation in Ca2+]i from 52.5±9.5 nM (mean±S.D., n=18) to 171.0±15.6 nM in cells between 15 and 25 μm in diameter. The depletion of extracellular Ca2+ diminished the Ca2+ elevation. The Na+-free condition also diminished the response. We concluded that the heat stimulation opens nonselective cation channels in putative warm cells from DRG neurons. |
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Keywords: | Calcium Thermosensitive neuron Fura-PE3 Dorsal root ganglion Warm cell |
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