| 油酸-脂多糖介导大鼠急性肺损伤及肺组织AP-1活性的变化 |
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| 引用本文： | 李琦,钱桂生,张青,汤正才,高正今,范亚川.油酸-脂多糖介导大鼠急性肺损伤及肺组织AP-1活性的变化[J].基础医学与临床,2002,22(1):70-73. |
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| 作者姓名： | 李琦 钱桂生 张青 汤正才 高正今 范亚川 |
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| 作者单位： | 第三军医大学,新桥医院,全军呼吸病研究所,重庆,400037 |
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| 基金项目： | 国家自然科学基金 (39730 2 1 0 ) |
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| 摘 要： | 本文探讨了在油酸－内毒素（OA＋LPS）介导的大鼠急性肺损伤（ALI）模型肺组织中，转录因子AP－1活性变化的意义。有用OA＋LPS序贯性两次致伤，复制ALI大鼠模型；采用凝胶迁移率分析法（EMSA）检测肺组织AP－1活性。结果显示：OA＋LPS模型组大鼠呼吸窘迫，PaO2早期低于8kPa，死亡率升高，肺含水量显著增加，肺水肿、浸润等病变严重，伴透明膜形成，肺组织AP－1活性显著升高；并且，间隔4h两次致伤后，P aO2降低幅度最大，死亡率高达78.57%，肺含水量最大，肺病理改变最重，肺组织AP－1活性最高。结果表明：OA＋LPS序贯性两次致伤，可以介导大鼠发生严重的ALI；ALI的发生、发展与AP－1活性异常升高有关。
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| 关 键 词： | 急性肺损伤 激活蛋白－1 ARDS EMSA |
| 文章编号： | 1001-6325(2002)01-0070-04 |
| 修稿时间： | 2001年4月27日 |
Changes of the AP-1 activity of lung in rats with acute lung injury induced by olic acid and lipopolysaccharide |
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| LI Qi,QIAN Gui sheng,ZHANG qing,et al.Changes of the AP-1 activity of lung in rats with acute lung injury induced by olic acid and lipopolysaccharide[J].Basic Medical Sciences and Clinics,2002,22(1):70-73. |
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| Authors: | LI Qi QIAN Gui sheng ZHANG qing |
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| Abstract: | Objective:To investigate the AP 1 activity in lung of rats with acute lung injury(ALI) induced by olic acid(OA)and lipopolysaccharide(LPS).Wistar rats were injured with OA(0.2ml/kg)and LPS(2mg/kg)to set up the ALI model.Electrophoretic mobility shift assays(EMSAs)were used to measure the AP 1 activity in lung of rats.Results showed that the ALI was stimulated in rats with OA+LPS, OA+LPS might cause the significant increase the AP 1 activity in lung of rats,especially in OA+LPS/4h group.Conclusion:1.OA+LPS may cause ALI in rats.2.The initiation and development of ALI may be explained by the obvious increase of AP 1 activity. |
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| Keywords: | acute lung injury activator puotein 1 olic acid lipopolysaccharide |
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