| 免疫酶细胞化学法测定JEV对C6136伊蚊细胞的附着 |
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| 引用本文： | 刘利兵,姜绍谆,马文煜,边江红.免疫酶细胞化学法测定JEV对C6136伊蚊细胞的附着[J].细胞与分子免疫学杂志,1994(2). |
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| 作者姓名： | 刘利兵 姜绍谆 马文煜 边江红 |
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| 作者单位： | 第四军医大学微生物学教研室 |
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| 摘 要： | 本文建立了测定病毒附着易感细胞的单克隆抗体免疫酶细胞化学法。用该法测定了ＪＥＶ对Ｃ６／３６伊蚊细胞的附着，结果表明ＪＥＶ能附着于Ｃ６／３６伊蚊细胞表面，提示伊蚊细胞表面可能具有ＪＥＶ受体，其表达可能与细胞的生长和分化状态有关。固定剂对细胞受体的活性有一定影响。
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| 关 键 词： | 抗体，单克隆 免疫酶细胞化学 日本乙型脑炎病毒 受体 |
Study of Attachment of JEV to C6/36 Cells by Immunoenzyme Cytochemical Assaay |
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| Lin Libing,Jiang Shaozhun,Ma Wenyu,Bian Jianghong.Study of Attachment of JEV to C6/36 Cells by Immunoenzyme Cytochemical Assaay[J].Journal of Cellular and Molecular Immunology,1994(2). |
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| Authors: | Lin Libing Jiang Shaozhun Ma Wenyu Bian Jianghong |
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| Abstract: | We established an immuno-enzyme cytochemical assay using monoclonal antibodies to detect attachment of Japanese encephalitis B virus(JEV)to C6/36 mosquito cells. Our results showed that JEV was able to attach to the surface of C6/36 cells, suggesting that there might be JEV--receptors on the cell surface,the expression of which seemed to be related with the growth and differentiation state of the cells. We also found that fixtive had some effects on the activating of the receptors. |
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| Keywords: | antibody monoclone immuno-enzyme cytochemical assay Japanese ence phalitis B yiruses receptor |
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