人参皂苷Rb1抑制自体静脉移植物内膜平滑肌细胞增生的研究

目的:研究人参皂苷Rb1通过抑制自体静脉移植物增殖细胞核抗原(PCNA)的表达,进而抑制移植物内膜平滑肌细胞过度增生的作用。方法:45只新西兰大白兔随机分为实验组、模型组、对照组,每组15只。应用no-touch外科技术获取颈外静脉后,采用外翻连续缝合方法将颈外静脉吻合至颈总动脉,建立静脉移植桥的动物模型。4周后利用苏木精-伊红染色观察移植静脉血管内膜形态及厚度变化,RT-PCR检测静脉移植血管中PCNA mRNA的表达。结果:光镜下结果显示:移植4周后,实验组、模型组和对照组移植血管的内膜厚度分别为(41.57±2.43)、(73.76±7.83)和(11.38±0.71)μm,各组间差异有统计学意义(P<0.05),移植静脉内膜/中膜厚度比分别为(1.21±0.09)、(1.44±0.12)和(0.28±0.07),各组间差异有统计学意义(P<0.05);移植4周后的实验组、模型组和对照组PCNA mRNA相对含量比值分别是(0.942±0.004)、(0.756±0.003)和(0.574±0.002),各组间差异有统计学意义(P<0.05)。结论:人参皂苷Rb1能够抑制移植血管PCNA mRNA的过度表达,进而有效减轻移植血管内膜增生导致的再狭窄,延长静脉血管桥的使用寿命。

Research of ginsenoside Rb1 on inhibition of intimal smooth musclecell hyperplasia in autologous vein grafts

Objective:To study the role of ginsenoside Rb1 in inhibiting the autologous vein graft proliferating cell nuclear antigen(PCNA) expression and autologous vein graft intimal hyperplasia. Method:Fourty-five New Zealand rabbits were randomly divided equally into experimental group,model group and control group.Endoscopic surgery was utilized to excise their external jugular veins,and then establish an animal model for transplanted vein bridges by anastomosing every unilateral jugular vein end-to-end with the common carotid artery.After 4 weeks,the morphology and thickness of the vein graft intimas were observed under HE staining;the PCNA mRNA expression was examined by RT-PCR. Result:The HE-stained sections under light microscopy showed that at 4 weeks after transplantation the thickness of vein graft intimas in experimental group,model group and control group were(41.57±2.43)μm,(73.76±7.83)μm,(11.38±0.71)μm,with significant differences(P<0.05);The intimal/medial thickness ratios of the 3 groups were(1.21±0.09),(1.44±0.12) and(0.28±0.07),and there were significantly differences(P<0.05).The relative expression coefficients of PCNA mRNA in experimental group,model group and and control group were(0.942±0.004),(0.756±0.003) and(0.574±0.002),and there were significantly differences(P<0.05). Conclusion:Ginsenoside Rb1 inhibits the overexpression of PCNA mRNA in vein grafts,which effectively reduces the risk of restenosis caused by intimal hyperplasia,and extends the lifespan of vein grafts.