电针对高脂血症合并脑缺血大鼠室管膜下区神经干细胞分化的影响

目的:观察电针后高脂血症合并脑缺血模型大鼠缺血侧脑室室管膜下区(SVZ)波形蛋白、β-微管蛋白(Tuj-1)表达的变化,研究针刺对神经干细胞的分化作用。方法:雄性SD大鼠72只,随机分为正常对照组、高脂血症模型组、高脂血症治疗组、脑缺血模型组、脑缺血治疗组、高脂血症合并脑缺血模型组、高脂血症合并脑缺血治疗Ⅰ组、高脂血症合并脑缺血治疗Ⅱ组,每组9只。采用经典高脂饲料喂养及FeCl3化学诱导大脑中动脉血栓闭塞制造高脂血症合并脑缺血模型。高脂血症治疗组电针"三阴交""丰隆"穴;脑缺血治疗组针刺"百会""水沟"穴;合并模型治疗Ⅰ组先电针"三阴交""丰隆",每日1次,治疗10 d后,造成脑缺血模型,再针刺"百会""水沟"穴;合并模型治疗Ⅱ组,脑缺血后针刺"百会""水沟"和电针"三阴交""丰隆"穴,每日1次,治疗7 d。治疗结束后,应用免疫组化方法检测大鼠前脑缺血侧SVZ波形蛋白、Tuj-1的表达变化。结果:波形蛋白、Tuj-1在正常和高脂血症脑组织表达较少,脑缺血后缺血侧SVZ中波形蛋白、Tuj-1的表达增强(P0.01),脑缺血治疗组波形蛋白、Tuj-1表达增强(P0.01),合并模型组表达远远弱于脑缺血组(P0.01),合并模型治疗组表达高于合并模型组(P0.01),治疗Ⅰ组各部位波形蛋白、Tuj-1的表达比治疗Ⅱ组要强(P0.01)。结论:高脂血症降低了脑缺血缺血侧SVZ神经干细胞的分化,针刺可促进高脂血症合并脑缺血后缺血侧SVZ神经干细胞的分化。在高脂血症阶段介入治疗,可通过促进脑缺血后缺血侧神经干细胞的分化,从而促进神经元的修复和再生。

Effect of electroacupuncture on proliferation and differentiation of neural stem cells in the subependymal zone of the cerebral lateral ventricle in rats with hyperlipemia and cerebral ischemia

Objective To observe the effects of electroacupuncture(EA) on the expression of vimentin and β-Tubulin(Tju-1) in the dorsolateral extension and the lateral wall of the lateral ventricle(both of which belong to the subependymal ventricular zone,SVZ) on the ischemic side of the brain in rats with hyperlipemia(HL) plus cerebral ischemia(CI) so as to study its underlying mechanism in relievting HL,CI and combined HL and CI by regulating the proliferation and differentiation of neural stem cells.Methods Seventy two male SD rats were randomized into control,HL,HL+ EA,CI,CI+manual acupuncture(MA),HL+CI,HL+CI+EAⅠ and HL+CI+EAⅡgroups,with 9 cases in each.HL model was established by feeding the animals with high fat forage for 6 weeks and CI model was duplicated by occlusion of the unilateral middle cerebral artery induced by regional application of FeCl_3.For rats of HL+EA group,EA(15 Hz,1-3 mA) was applied to bilateral "Sanyinjiao"(SP 6) and "Fenglong"(ST 40) for 20 min,once daily for 17 days.For rats of CI+MA group,"Baihui"(GV 20) and "Shuigou"(GV 26) were punctured and stimulated manually for 1 min(the former with the needle retained for 20 min),once daily for 7 days.For rats of HL+CI+EAⅠ group,bilateral "Sanyinjiao"(SP 6) and "Fenglong"(ST 40) were stimulated electrically after needle-insertion,once daily for 10 days,followed by duplicating CI model and performing MA stimulation of GV 20 and GV 26 respectively,once daily for 7 days.For rats of HL+CI+EAⅡgroups,EA was applied to bilateral SP 6 and ST 40,and simultaneous MA stimulation of GV 20 and GV 26,once daily for 7 days after HL+CI.The expression of vimentin and Tuj-1 in the dorsolateral extension and the lateral wall of the cerebral lateral ventricle on the ischemic side was detected by immunohistochemistry.Results Compared with the control group,the expression levels of both vimentin and Tuj-1 in the dorsolateral extension and the lateral wall of the lateral ventricle of the brain on the ischemic side were increased significantly in the CI and HL+CI groups(P<0.01),while compared with the CI group,the expression of vimentin and Tuj-1 in the CI+MA group were increased further significantly(P<0.01).In comparison with the HL+CI group,the expression of vimentin and Tuj-1 in the HL+CI+EAⅠ and HL+CI+EAⅡ groups were also upregula-ted considerably(P<0.01).Comparison between the HL+CI+EAⅠ and HL+CI+EAⅡ groups showed that the expression le-vels of vimentin and Tuj-1 were significantly lower in the latter group than in the former group(P<0.01).No significant diffrences were found between the HL and control groups,and between the HL and HL+EA groups in the expression of vimentin and Tuj-1(P>0.05).Conclusion Acupuncture and Acupuncture+EA can further significantly upregulate the expression levels of vimentin and Tuj-1 in both the dorsolateral extension and the lateral wall of the cerebral lateral ventricle on the ischemic side in rats with CI and CI+HL,which may contribute to their effects in improving CI and HL+CI by suppressing the reduction of proliferation and differentiation of neural stem cell in the SVZ of the ischemic brain induced by CI and HL+CI.