| 桃儿七组织培养体系的建立及鬼臼毒素的检测 |
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| 引用本文： | 栗孟飞,李唯. 桃儿七组织培养体系的建立及鬼臼毒素的检测[J]. 中草药, 2010, 41(8): 1366-1370 |
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| 作者姓名： | 栗孟飞 李唯 |
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| 作者单位： | 甘肃农业大学生命科学技术学院，甘肃 兰州 730070;甘肃农业大学生命科学技术学院，甘肃 兰州 730071 |
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| 基金项目： | 高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的建立桃儿七组织培养体系及其鬼臼毒素的提取检测方法。方法以桃儿七成熟分离胚为材料,研究了不同浓度激素、活性炭(AC)对无菌苗诱导的影响;以桃儿七无菌苗切除后约2.0 cm的根茎为材料,考察不同浓度激素对根继代及生长发育的影响,并定期对继代根、叶(叶柄)及培养基进行鬼臼毒素的提取及HPLC法检测。结果分离胚在MS+2.0%蔗糖+0.4%琼脂+1.0 mg/mL GA3+1.0 g/L AC的培养基培养20 d后,无菌苗诱导率为92.0%,无菌苗生长发育良好;切除后约2 cm的根茎接种在MS+2.0%蔗糖+0.4%琼脂+1.0 g/LAC+1.0 mg/mLIBA继代40 d后,根长11.59 cm,植株生长发育良好;对继代根、叶(叶柄)及培养基进行鬼臼毒素的提取及HPLC法检测显示,鬼臼毒素量大小依次为根培养基叶(叶柄),50 d后分别为33.17、12.97、4.55μg/mL。结论本研究建立了桃儿七组织培养体系,证明了利用桃儿七根培养生产鬼臼毒素的可行性。
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| 关 键 词： | 桃儿七 根继代 鬼臼毒素 HPLC |
| 收稿时间： | 2009-11-15 |
EstablishmenTof tissue culture system of Sinopodophy emodi and identification of podophyllotoxin |
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| LI Meng-fei and LI Wei. EstablishmenTof tissue culture system of Sinopodophy emodi and identification of podophyllotoxin[J]. Chinese Traditional and Herbal Drugs, 2010, 41(8): 1366-1370 |
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| Authors: | LI Meng-fei and LI Wei |
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| Affiliation: | College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | establish the tissue culture system of Sinopo dophyllum emodi and the extraction and ident if ication methods of poophyllo Tox in. Methods the effects of seedling induced rat by different concentr ations of hormones and activ ated charcoal ( AC) were studied, taking the separated mature embryo as material. |
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| Keywords: | Sinopodophyllum emodi (Wall.) Ying root sub-culture podophyllotoxin HPLC |
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