| 绞股蓝RAPD标记及序列分析 |
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| 引用本文： | 周娟,罗育,吴耀生,李科志,徐鹏,赵瑞强.绞股蓝RAPD标记及序列分析[J].中草药,2010,41(10):1692-1696. |
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| 作者姓名： | 周娟 罗育 吴耀生 李科志 徐鹏 赵瑞强 |
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| 作者单位： | 广西医科大学 生物化学与分子生物学教研室，广西 南宁 530021;广西医科大学 生物化学与分子生物学教研室，广西 南宁 530022;广西医科大学 生物化学与分子生物学教研室，广西 南宁 530023;广西医科大学 生物化学与分子生物学教研室，广西 南宁 530024 |
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| 基金项目： | 广西科学基金资助项目，广西区教育厅硕士研究生科研创新项目 |
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| 摘 要： | 目的利用绞股蓝RAPD标记有效鉴别绞股蓝及其伪品。方法用19条引物对17个绞股蓝样品和一个乌蔹莓样品进行RAPD-PCR扩增,并对绞股蓝DNA特异条带克隆测序及序列分析。结果获得一个绞股蓝RAPD标记。经克隆测序,8个序列高度保守,片段长度在766~770 bp,相似性均在97%以上。结论获得的RAPD标记可以作为合成探针的基础,用于鉴别绞股蓝及其伪品。
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| 关 键 词： | 绞股蓝 RAPD标记 序列分析 |
| 收稿时间： | 2010/5/23 0:00:00 |
Sequence analysis of RAPD markers related to Gynostemma pentaphyllum |
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| ZHOU Juan,LUO Yu,WU Yao-sheng,LI Ke-zhi,XU Peng and ZHAO Rui-qiang.Sequence analysis of RAPD markers related to Gynostemma pentaphyllum[J].Chinese Traditional and Herbal Drugs,2010,41(10):1692-1696. |
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| Authors: | ZHOU Juan LUO Yu WU Yao-sheng LI Ke-zhi XU Peng and ZHAO Rui-qiang |
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| Institution: | Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective The study is to identify the medicinal plant Gynostemma pentaphyllum and its fake called Cayratia japonica through the RAPD markers.Methods Nineteen random primers screened were applied to amplifying the total DNA extracts of 18 samples with RAPD and the spectific fragments of G.pentaphyllum were analyzed by cloning and sequencing.Results A RAPD marker of G.pentaphyllum was acquired.The result of sequence analysis indicated that the length of eight sequences is 766-770 bp and the identities of them are over 97%.Conclusion The RAPD marker acquired could provide a basis for making probe to distinguish G.pentaphyllum and its spurious breed. |
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| Keywords: | Gynostemma pentaphyllum (Thunb ) Markino RAPD marker sequence analysis |
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