| 吴茱萸道地药材的高效液相指纹图谱研究 |
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| 引用本文： | 袁清照,张水寒,李顺祥,黄丹,蔡萍.吴茱萸道地药材的高效液相指纹图谱研究[J].中草药,2010,41(9):34-38. |
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| 作者姓名： | 袁清照 张水寒 李顺祥 黄丹 蔡萍 |
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| 作者单位： | 湖南省中医药研究院 中药新药研究与开发重点实验室，湖南 长沙 410013;湖南省中医药研究院 中药新药研究与开发重点实验室，湖南 长沙 410014;湖南省中医药研究院 中药新药研究与开发重点实验室，湖南 长沙 410015;湖南中医药大学 中药现代化实验室，湖南 长沙 410208;湖南省中医药研究院 中药新药研究与开发重点实验室，湖南 长沙 410015;湖南中医药大学 中药现代化实验室，湖南 长沙 410209;湖南省中医药研究院 中药新药研究与开发重点实验室，湖南 长沙 410013 |
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| 基金项目： | 科学技术部国家"十一五"科技支撑计划课题，国家自然科学基金资助项目，湖南省科学技术厅科技资助项目 |
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| 摘 要： | 目的建立吴茱萸道地药材化学特征指纹图谱。方法采用HPLC梯度洗脱法,对吴茱萸药材进行测定,流动相为0.04%辛烷磺酸钠与0.05%磷酸的水溶液-乙腈,检测波长230 nm,记录80 min的色谱。结果运用梯度洗脱能很好分离吴茱萸的各类成分,经方法学考察,HPLC指纹图谱具有良好的重现性;11批样品中21个特征指纹峰有专属性。结论本研究建立的指纹图谱分析方法可用于吴茱萸药材质量的综合评价,也可用于道地性考察。
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| 关 键 词： | 吴茱萸 HPLC 道地性 指纹图谱 |
| 收稿时间： | 1/5/2010 12:00:00 AM |
Study on HPLC fingerprint of genuine medicinal materials of Evodiae Fructus |
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| YUAN Qing-zhao,ZHANG Shui-han,LI Shun-xiang,HUANG Dan and CAI Ping.Study on HPLC fingerprint of genuine medicinal materials of Evodiae Fructus[J].Chinese Traditional and Herbal Drugs,2010,41(9):34-38. |
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| Authors: | YUAN Qing-zhao ZHANG Shui-han LI Shun-xiang HUANG Dan and CAI Ping |
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| Institution: | Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;Key Laboratory of Modernization of Chinese Medicine, Hunan Universityof Chinese Medicine, Changsha 410208, China;Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;1. Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;2. Key Laboratory of Modernization of Chinese Medicine, Hunan Universityof Chinese Medicine, Changsha 410208, China |
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| Abstract: | Objective To establish the HPLC fingerprint of Evodiae Fructus from genuine medicinal materials.Methods The gradient elution was adopted with acetonitrile 0.04% octane sulfuric acid sodium salt and 0.05% phosphoric acid in water as mobile phase,and detection wavelength was at 230 nm.All the samples were recorded in 80 min.Results The constitutents of Evodiae Fructus were well separated on HPLC,and the HPLC fingerprint of 11 batches of samples was established.The results showed that this method had a good repeatability,and the 21 common peaks had exclusive properties.Conclusion The method developed can be used to evaluate the quality of Evodiae Fructus synthetically and examine the genuineness. |
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| Keywords: | Evodiae Fructus HPLC authentic herb fingerprint |
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