高效液相色谱法测定胃力片中柚皮苷和橙皮苷的含量

目的建立HPLC法测定胃力片中柚皮苷和橙皮苷的含量。方法采用HPLC法,色谱柱为Diamonsil C18（4.6mm×150mm,5μm）,以甲醇-1%冰醋酸水溶液（32∶68,v/v）为流动相,流速为1.0mL.min-1,检测波长为283nm。结果柚皮苷与橙皮苷线性范围分别为14.0-126.0μg.mL^-1（r=0.9997）、2.88-25.92μg.mL^-1（r=0.9997）,平均回收率分别为96.4%、97.8%。结论该方法简便准确、重现性好、可为胃力片质量控制提供依据。

Determination of narigin and hesperidin in Weili tablets by HPLC

Objective To establish an HPLC method for the determination of narigin and hesperidin in Weili tablets.Methods The HPLC system consisting of an ODS column（4.6 mm×150 mm,5 μm）and a mixture of methanol and 1% acetic acid-water（32∶68,v/v）as the mobile phase was adopted.The flow rate was 1.0 mL·min^-1 and the UV detection was 283 nm.Results The assay displayed good linearity over 14.0～126.0 μg·mL^-1（r=0.999 7）and 2.88～25.92 μg·mL^-1（r= 0.999 7）,respectively.The average recoveries for narigin and hesperidin were 96.4% and 97.8%,respectively.Conclusion The method is simple and accurate,and can be used for the quality control of Weili tablets.