铁甲草抑制二甲基亚硝胺诱导大鼠肝纤维化的实验研究

目的 观察铁甲草醇提取物抑制二甲基亚硝胺诱导大鼠肝纤维化形成的作用效果并探讨其作用机制.方法 采用二甲基亚硝胺诱导的大鼠肝纤维化模型,用高、中、低剂量(12.6,4.2,1.4 g/kg)的铁甲草醇提液进行干预治疗,并与秋水仙碱进行对照,检测大鼠肝功能、血清透明质酸(HA)、层粘蛋白(LN)和Ⅲ型前胶原氨基端肽(PCⅢ)、 肝组织羟脯胺酸(Hyp)含量及肝脏病理组织学改变. 结果 铁甲草高、中、低剂量组血清谷丙转氨酶(ALT)及铁甲草高剂量组血清谷草转氨酶(AST)水平与模型组比较有统计学意义(P <0.05);铁甲草各剂量组大鼠肝组织Hyp含量均明显低于模型组(P <0.05).铁甲草各剂量组大鼠血清HA 、LN、 PCⅢ含量明显低于模型组,差异有统计学意义(P<0.05).铁甲草高、中、低剂量组肝组织纤维化病变明显减轻,与模型组比较,差异有统计学意义(P <0.05). 结论 铁甲草对二甲基亚硝胺诱导的大鼠肝纤维化形成具有明显的抑制作用,其机制可能与保护肝细胞和抑制胶原纤维合成有关.

Inhibitive Effects of Extract of Cassia mimosoides Linn on Dimethylnitrosamine-induced Hepatic Fobrosis in Rats

Objective The aim of this study was to observe the inhibitive effects of the extract of Cassia mimosoides linn, on dim-ethylnitrosamine-induced hepatic fibrosis in rats and to explore the action mechanism. Methods Intervention treatment with the extract of Cassia mimosoides Linn, was explored in the rats with dimethylnitrosamine-induced hepatic fibrosis at the high, intermediate, and low doses of 12.6 g/kg,4.2 g/kg,and 1.4 g/kg respectively, compared with the group treated with colchicine. Hepatic function, levels of serum hyaluronic acid (HA) , serum laminin (LN) , serum aminoterminal propeptide of type III procolla-gen (PIIINP) , hepatic hydroxyproline (Hyp) in rats were assayed and histopathological alterations in rats were examined. Results The results indicated that, compared with those in the model group, alterations in the levels of serum alanine aminotrans-ferase (ALT) in the groups treated with extract of Cassia mimosoides at low, intermediate, and high doses, and serum aspartate aminotransferase ( AST) level in the group at the high dose were statistically significant (P<0.05 ) ; hepatic hydroxyproline ( Hyp) levels in the rats treated with extract of Cassia mimosoides at various doses were significantly lower than those in the model group (P<0.05 ). Levels of serum hyaluronic acid ( HA) , serum laminin ( LN) , and serum aminoterminal propeptide of type III procollagen (PIIINP) in the groups treated with extract of Cassia mimosoides were significantly lower than those in the model group, and the differences were statistically significant (P<0.05). Hepatic fibrosis of rats in the groups treated with extract of Cassia mimosoides at high, intermediate, and low doses alleviated significantly, and the differences compared with the pathological changes in the model group were statistically significant. Conclusion It was concluded that, intragastric administration with extract of Cassia mimosoides showed significant inhibitive effects on the dimethylnitrosamine -induced hepatic fibrosis in rats, and the inhibitive mechanisms might be related with the protective effects on hepatic cells and the inhibition on collagen fiber synthesis.