神经节苷脂GM-1对脑缺血再灌注大鼠诱导型-氧化氮合成酶的影响

目的:研究单唾液酸四已糖神经节苷脂(GM-1)对脑缺血再灌注后诱导型一氧化氮合成酶(iNOS)的影响并探讨其可能机制.方法:采用线栓法制作大鼠大脑中动脉闭塞模型,缺血2 h再灌注22 h.将雄性SL大鼠随机分成假手术组,模型对照组和GM-1处理组(15、30、60 mg/kg 3个给药剂量组).通过红四氮唑(TTC)染色和图像分析计算各组脑梗死体积,采用生物化学法测量各组伤侧脑中NO含量和iNOS活性.结果:与模型对照组相比,GM-1中、高剂量处理组脑梗死体积缩小,iNOS活性和NO量下降,低剂量组变化不明显.结论:脑缺血后iNOS升高与脑损伤关系密切,而GM-1可以对抗iNOS和NO上升,并减轻脑缺血损伤.

Effect of gangliaside GM1 on induced nitric oxide synthase after focal cerebral ischemical reperfusion in rats

Objective:To study the effect of gangliaside GM-1 on induced nitric oxide synthase(iNOS) after cerebral ischemic reperfusion and approach its possible mechanism. Methods:Using 2 h ischemia and 22 h reperfusion middle cerebral artery occlusion model,male SD rats were randomly divided into sham-operated group,model control group and GM-1 treat group which received GM-1 at doses of 15,30 or 60 mg/kg. The infarct size was evaluated by 2,3,5-triphenyltetrazolium chloride(TTC) staining and image assay. The NO content and the activity of induced nitric oxide synthase(iNOS) were determined by biochemical assay. Results:In comparison with model control group,the infarct size,iNOS activity and NO content were decreased in the groups receiving GM-1 at both middle and high dose except low dose. Conclusion:The rise of iNOS activity could correlate closely with brain injury,and GM-1 could reduce iNOS activity,NO connect and brain injury after cerebral ischemia.