高效液相色谱法考察复方头孢氨苄胶囊的溶出度

采用反相高效液相色谱外标法，对复方头孢氨苄胶囊溶出度进行考察。方法：采用转篮法，以4％乙酸溶液为溶剂，转速为100r/min，时间为45min。色谱条件：C18反相谱柱为固定相，V（水）：V（甲醇）：V(3．86％乙酸钠溶液）：V(4％乙酸溶液）＝742：240：15：3为流动相，流速为1.2mL/min，紫外检测波长为254nm,在此条件下头孢氨苄淀两组分能很好地分离。本法的线性范围分别是头孢氨苄为：27．6－276μg/mL，甲氧苄啶为：5．56－ 55．6μg/mL，平均回收率为头孢氨苄：99．32％，RSD＝0．78％；甲氧苄啶：100．1％，RSD＝0．57％。

Determination of dissolution of compound cephalexin capsule by HPLC

A reversed-phase HPLC (RP-HPLC) method for the determination of the dissolution of compound cephalexin capsule was developed. A basket stirring technique was applied, with 4% acetic acid solution as dissolvent, at speed of 100 r/min for 45 min . A C18 reversed-phase column was used as fixed phase and water-methanol-3.86% sodium acetate-40% acetic acid (742∶240∶15∶3) as mobile phase. The flow rate was 1.2 ml/min and UV detection was performed at 254 nm. Cephalexin and Trimethoprim (TMP) could be separated in this condition. The linear ranges were 27.6～276 μg/mL for Cephalexin and 5.56～55.6 μg/mL for TMP. The recovery and RSD were 99.32% and 0.78% for Cephalaxin and 100.1% and 0.57% for TMP respectively.