Effects of naphthoquinone on airway responsiveness in the presence or absence of antigen in mice |
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Authors: | Ken-ichiro Inoue Hirohisa Takano Takamichi Ichinose Shigeo Tomura Rie Yanagisawa Miho Sakurai Daigo Sumi Arthur K Cho Kyoko Hiyoshi Yoshito Kumagai |
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Institution: | (1) Environmental Health Sciences Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba Ibaraki, 305-8506, Japan;(2) Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan;(3) Department of Health Science, Oita University of Nursing and Health Science, Oita, Japan;(4) Department of Environmental Medicine, Institute of Community Medicine, University of Tsukuba, Tsukuba, Japan;(5) Southern California Particle Center and Supersite, University of California, Los Angeles, USA |
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Abstract: | We have recently demonstrated that naphthoquinone (NQ), one of extractable chemical compounds of diesel exhaust particles
(DEP), enhances antigen-related airway inflammation with goblet cell hyperplasia in mice (Inoue et al. in Eur Respir J 209(2):259–267,
2007). Further, NQ has enhanced lung expressions of interleukin (IL)-4 and IL-5. However, the effects of NQ on other cardinal
features of asthma have not been completely investigated. The aim of the present study was to evaluate the effects of NQ on
airway responsiveness on the model. Vehicle, NQ, ovalbumin (OVA), or NQ + OVA was administered intratarcheally to ICR mice
for 6 weeks. Twenty-four hours after the last instillation, lung histology, lung functions such as total respiratory system
resistance (R) and Newtonian resistance (R
n), and protein level of IL-13 and mRNA level for MUC5AC in the lung were examined. Repetitive exposure to NQ aggravated antigen-related
lung inflammation. NQ alone enhanced R and R
n as compared to vehicle without statistical significance. OVA alone or NQ plus OVA showed increases in R and R
n, which was prominent in NQ plus OVA (P < 0.05 vs. vehicle). Combined exposure to NQ and OVA elevated the levels of IL-13 and MUC5AC in the lung as compared with
exposure to NQ or OVA alone. These results indicate that NQ can enhance airway hyperresponsiveness in the presence or absence
of an antigen. Also, amplified lung expressions of IL-13 and MUC5AC might partly contribute to the deterioration of asthma
features by NQ. |
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Keywords: | Naphthoquinone Antigen Airway hyperresponsiveness MUC5AC |
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