| 促红细胞生成素改善实验性变态反应性脑脊髓炎症状观察 |
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| 引用本文： | 张祥,李琴英,肖保国. 促红细胞生成素改善实验性变态反应性脑脊髓炎症状观察[J]. 中国免疫学杂志, 2010, 26(4). DOI: 10.3969/j.issn.1000-484X.2010.04.009 |
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| 作者姓名： | 张祥 李琴英 肖保国 |
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| 作者单位： | 复旦大学附属华山医院神经病学研究所,上海,200040 |
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| 摘 要： | 目的:观察促红细胞生成素(EPO)对实验性变态反应性脑脊髓炎(EAE)的神经保护作用。方法:制备EAE小鼠模型,对EPO干预组和EAE对照组小鼠进行临床症状及病理组织学评估;提取制备外周及中枢浸润淋巴细胞,应用流式细胞仪观察CD4+T亚群、炎性细胞因子水平以及细胞死亡情况;应用LDH测定评估不同浓度EPO对外周淋巴细胞死亡的影响。结果:EPO可改善EAE小鼠临床症状和病灶局部淋巴细胞浸润(P0.05);EPO干预组小鼠中枢浸润细胞以及外周淋巴细胞CD4+T细胞比例下降,IL-17和IFN-γ等炎性因子水平降低(P0.05),而细胞死亡比例以及IL-10、CD4+CD25+调节性T细胞水平与EAE对照组无显著差异(P0.05);1~1 000 U/ml浓度范围内EPO对外周单核淋巴细胞LDH的释放无显著影响(P0.05)。结论:EPO可以通过减少病灶炎性细胞浸润以及抑制IL-17和(或)IFN-γ水平等途径,对EAE具有神经保护作用。
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| 关 键 词： | 促红细胞生成素 实验性变态反应性脑脊髓炎 神经保护 |
Erythropoietin ameliorate experimental allergic encephalomyelitis |
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| ZHANG Xiang,LI Qin-Ying,XIAO Bao-Guo. Erythropoietin ameliorate experimental allergic encephalomyelitis[J]. Chinese Journal of Immunology, 2010, 26(4). DOI: 10.3969/j.issn.1000-484X.2010.04.009 |
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| Authors: | ZHANG Xiang LI Qin-Ying XIAO Bao-Guo |
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| Affiliation: | ZHANG Xiang,LI Qin-Ying,XIAO Bao-Guo.Institute of Neurology,Huashan Hospital,Fudan University,Shanghai 200040,China |
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| Abstract: | Objective:To investigate the beneficial effect of erythropoietin(EPO) on experimental allergic encephalomyelitis(EAE).Methods:The severity of EAE mice with or without EPO therapy were evaluated through clinical symptoms score and histological observation.Mononuclear cells from spleens,lymph nodes,brains and lumber spinal cords were applied to flow cytometry to detect ratios of CD4+T subgroup,scales of apoptotic and dead cells,levels of inflammatory cytokines.LDH release of peripheral mononuclear cells were ... |
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| Keywords: | Erythropoietin Experimental allergic encephalomyelitis Neuroprotecion |
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