三分类血细胞分析仪的筛查与镜检标准的制定

目的：评价Coulter三分类血细胞分析仪对门、急诊患者的筛查效果；以及血涂片镜检标准的制定对三分类血细胞分析仪的补充作用。方法：对60例门、急诊患者的血液标本同时进行CoulterA^c．T diff^TM及Sysmex XE-2100血细胞分析仪全血细胞分析；对76例门、急诊患者的血液标本同时进行Coulter A^c．Tdiff^TM三分类及人工分类。结果：Coulter A^c．T diff^TM与Sysmex XE-2100血细胞分析仪全血细胞分析，包括WBC、RBC、Hb及Pit两者相关性较好（r〉0．90）；Coulter A^c．T diff^TM三分类与人工分类结果比较，中性粒细胞镜检法明显低于仪器法（P〈0．001），淋巴细胞镜检法明显高于仪器法（P〈0．001），中间细胞两种方法差异无显著性（P〉0．05）；中性粒细胞和淋巴细胞仪器法与镜检法相关性良好（r〉0．95），而中间细胞两者不相关（r〈0．1）。结论：使用三分类血细胞分析仪进行全血细胞分析包括WBC、RBC、Hb及Pit结果可信，可提高工作质量和效率；而三分类血细胞分析仪对白细胞分类的筛查作用有限，必须制定严格的显微镜复检标准，才能防止血液病患者的漏诊或误诊。

Screening Test of 3-differentiation Blood Cell Analyzer and Criteria for Performing Peripheral Blood Smear Review

Objective:To evaluate the screening test of 3-differentiation blood cell analyzer and make the criteria for performing peripheral blood smear review in out-patients and emergency patients.Methods:60 blood smaples in out-patients and emergency patients were determined by Sysmex XE-2100 and CoulterA~C.T diff~(TM) hematology analyzer.As well as,76 blood samples in out-patients and emergency patients were detected by CoulterA~C.T diff~(TM) blood cell analyzer and compared by microscopic examination.Results:Between 3-differential and 5-differential method,there were very good correlations in complete blood counts(CBC),including White Blood Cell(WBC),Red Blood Cell(RBC),Hemoglobin concentration(Hgb) and Platelet count(Plt),r>0.90.Nevertheless,the significant difference was observed in neutrophils and lymphocytes between the counting results of two methods.Manual analysis far surpassed automated analysis in lymphocytes,P<0.001,But automated analysis far surpassed manual analysis in neutrophils,P<0.001,No significant difference was observed in nononuclear,P>0.05.Between the manual and automatic method,there were very good correlations in neutrophils and lymphocytes(r>0.95),But there was a bad correlation in nononuclear,r<0.1.Conclusions:The results on complete blood counts(CBC) by 3-differentiation blood cell analyzer is accurate and reliable for screening test in out-patients and emergency patients,It can also improve the quality and efficiency.But the screening ability to abnormal morphology cells is limited,It is helpful to set up criteria with blood cell microscopic examination,for preventing from leaking and mistaking diagnosis of hematonosis.