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丙泊酚对全脑缺血/再灌注损伤大鼠保护作用研究
引用本文:宋春雨,陶涛,崔晓光,席宏杰,岳子勇,王楠. 丙泊酚对全脑缺血/再灌注损伤大鼠保护作用研究[J]. 国际麻醉学与复苏杂志, 2011, 32(4): 393-397. DOI: 10.3760/cma.j.issn.1673-4378.2011.08.003
作者姓名:宋春雨  陶涛  崔晓光  席宏杰  岳子勇  王楠
作者单位:黑龙江省哈尔滨医科大学附属第二临床医学院麻醉科&黑龙江省麻醉与危重病研究重点实验室,150086
基金项目:黑龙江省青年专项基金,哈尔滨市科委基金
摘    要:目的探讨丙白酚对大鼠全脑缺血/再灌注损伤(ischemia/reperfusion injury,I/RI)的保护作用。方法65只Wistar雄性大鼠采用完全随机法分为5组:假手术组(S组)、缺血/再灌注(ischemia/reperfusion,I/R)组(I/R)组、丙泊酚组1(P1)、丙泊酚组2(P2)、丙泊酚组...

关 键 词:脑缺血/再灌注损伤  丙泊酚  神经行为学  凋亡

The protective effect of propofol against global cerebral ischemia/reperfusion injury in rats
SONG Chun -yu,TAO Tao,CUI Xiao -guang,XI Hong -jie,YUE Zi -yong,WANG Nan. The protective effect of propofol against global cerebral ischemia/reperfusion injury in rats[J]. international journal of anesthesiology and resuscitation, 2011, 32(4): 393-397. DOI: 10.3760/cma.j.issn.1673-4378.2011.08.003
Authors:SONG Chun -yu  TAO Tao  CUI Xiao -guang  XI Hong -jie  YUE Zi -yong  WANG Nan
Abstract:Objective To investigate the protective effects of propofol on global cerebral ischemia-reperfusion injury (I/RI)in rats and the underlying mechanism. Methods 65 male Wistar rats weighting 250 g-300 g were randomly divided into 5 groups (n=13): group S sham operation, group ischemia/reperfusion (I/R), group P1 propofol (0.5 mg·kg1·min-1)+I/R, group P2 propofol (1.0 mg·kg-1·min-1)+I/R and group P3 propofol (1.5 mg·kg-1·min-1)+I/R. I/R group was induced by occlusion of bilateral common carotid arteries combined with controlled hypotension for 10 min. In group P1, P2and P3, propofol was continued intravenous infusion for 60 min before FR. The neurological behavior was evaluated at 6, 24, 48, 72 h, 4, 5, 6 d and 7 d after I/R. Rats were killed at 7 d in each group and the brains were removed for pathomorphologic examination of the survived pyramidal neurons in the area of CA1 hippocampus. Flow cytometric was applicated for detection apoptosis at 24 h of reperfusion in each group. Results The rats subjected to ischemic and reperfusion appeared behavioral defects. The behavior of animals was significantly better in group P2 and in groups P3 than that of in groups P1 and I/R. The HG scores of normal neurons in CA1 of hippocampus in S group, I/R group, P1 group, P2 group, P3 group was 0, 2.8±0.4, 2.6±0.5, 2.0±0.7, 1.4±0.5, respectively. The apoptosis rates in S group, I/R group, P1 group, P2 group, P3group was (6.5±0.6), (51.5±2.8), (48.2±0.6), (24.7±1.2), (18.6±0.8)%, respectively. Conclusion Propofol confers dose-dependent protection against cerebral I/R injury, and this effect may be involved in the anti-apoptosis effect of propofol.
Keywords:Ischemia/reperfusion injury  Propofol  Neurological behavior  Apoptosis
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