| 荧光定量PCR检测婴幼儿巨细胞病毒感染情况分析 |
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| 引用本文： | 李云,王惠萱,姜昌丽,范泉水.荧光定量PCR检测婴幼儿巨细胞病毒感染情况分析[J].国际检验医学杂志,2011,32(16):1841-1842,1844. |
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| 作者姓名： | 李云 王惠萱 姜昌丽 范泉水 |
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| 作者单位： | 1. 中国人民解放军成都军区昆明总医院检验科,昆明,650032
2. 中国人民解放军成都军区疾病预防控制中心军事医学研究所,昆明,650032 |
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| 摘 要： | 目的探讨荧光定量PCR(FQ-PCR)方法检测婴幼儿尿液人巨细胞病毒(HCMV)在HCMV感染诊断中的意义。方法采用FQ-PCR检测148例临床疑似HCMV感染婴幼儿尿液中HCMV-DNA水平。同时采用ELISA法检测婴幼儿血清HCMV-IgM抗体。在尿液HCMV-DNA检测阳性患儿中比较血清HCMV-IgM抗体阳性与阴性患儿尿液中HCMV-DNA拷贝数差异。结果 FQ-PCR检测尿液HCMV-DNA的阳性率为35.14%,ELISA检测婴儿血清HCMV-IgM的阳性率为15.54%。两种方法对HCMV感染诊断的阳性率差异有统计学意义(P<0.01)。在尿液HCMV-DNA检测阳性患儿中,IgM抗体阳性组尿液HCMV-DNA拷贝数显著高于阴性组(P<0.01)。结论 FQ-PCR检测尿液HCMV-DNA是早期诊断婴幼儿HCMV感染的敏感有效的方法。
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| 关 键 词： | 巨细胞病毒 聚合酶链反应 婴儿 感染 |
Detection of human cytomegalovirus infection in infants by fluorescent quantitative PCR |
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| Li Yun,Wang Huixuan,Jiang Changli,Fan Quanshui.Detection of human cytomegalovirus infection in infants by fluorescent quantitative PCR[J].International Journal of Laboratory Medicine,2011,32(16):1841-1842,1844. |
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| Authors: | Li Yun Wang Huixuan Jiang Changli Fan Quanshui |
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| Institution: | Li Yun1,Wang Huixuan1,Jiang Changli1,Fan Quanshui2 (1.Department of Clinical Laboratory,Kunming General Hospital of PLA,Kunming,Yunnan 650032,China,2.Military Medical Institute,Center for Control and Prevention of Diseases of Chengdu Military Command,China) |
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| Abstract: | Objective To explore the clinical significance of fluorescent quantitative PCR(FQ-PCR) detection of human cytomegalovirus(HCMV) in urine of infants for the diagnosis of HCMV infection.Methods HCMV in urine of infants with suspect diagnosis of HCMV infection was detected by FQ-PCR and anti-HCMV IgM antibody(HCMV-IgM) in serum was also detected by ELISA. Of infants positive with HCMV-DNA in urine,levels of HCMV-DNA was compared between infants positive or negative with HCMV-IgM.Results The positive rate of FQ... |
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| Keywords: | cytomeglovirus polymerase chain reaction infants infection |
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