凋亡抑制基因在急性白血病及骨髓增生异常综合征中的研究

目的探讨急性白血病及骨髓增生异常综合征患者骨髓凋亡抑制基因MCL-1、livin、aven的表达及临床意义。方法选取2012年9月~2013年12月本院收治的113例初发急性白血病患者,32例骨髓增生异常综合征患者及20例对照组患者（缺铁性贫血12例、血小板减少性紫癜8例）的新鲜骨髓液标本,采用RT-PCR方法检测MCL-1、livin、aven基因mRNA的表达水平及表达率。结果 AL组和MDS组MCL-1、livin、aven mRNA表达水平及阳性表达率分别高于对照组,差异有统计学意义（P〈0.05）。MCL-1与livin基因mRNA的表达呈正相关（r=0.586,P〈0.05）,MCL-1与aven基因的表达呈正相关（r=0.603,P〈0.05）,liven基因与aven基因表达无相关性（r=0,P〉0.05）。结论 3种凋亡抑制基因MCL-1、livin、aven基因mRNA在急性白血病及骨髓增生异常综合征中表达均增高。

The reserch of apoptosis suppressor gene in acute leukemia and myelodysplastic syndrome

Objective To study the expression of the apoptosis suppressor MCL-l,livin and aven in acute leukemia and myelodysplastic syndrome and its clinical significance.Methods 113 patients with newly diagnosed acute leukemia pa- tients were selected from September 2012 to December 2013 in our hospital,32 cases of myelodysplastic syndrome pa- tients and 20 cases of control group patients （12 patients with iron deficiency anemia,8 cases with thrombocytopenia purpura）,whose fresh bone marrow samples were collected.RT-PCR method was used for detection of MCL-l,livin,aven gene expression and mRNA expression.Results The mRNA expression and positive expression rate of MCL-l,livin, aven in AL group and MDS group was higher than that in control group respectively,with statistical difference （P〈0.05）. The expression of MCL-1 was positively correlated to the expression of livin （r=0.586,P〈0.05）,the expression of MCL-1 was positively correlated to the expression of aven （r=0.603,P〈0.05）,no correlation between the expression of livin and the expression of aven （r=0,P〉0.05）.Conclusion The expression of apoptosis suppressor MCL-1,livin and aven were up- regulated in aute leukemia and myelodysplastic syndrome.