Von Willebrand factor purification from human plasma cryoprecipitate |
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| Authors: | Dr Miryana Burnouf-Radosevich |
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| Affiliation: | (1) Unité de Recherche et Développement, Centre de Transfusion Sanguine de Lille, Lille, France;(2) R & D Unit, LFB-Lille, 59 Rue Trévise, F-59011 Lille, France |
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| Abstract: | Summary Von Willebrand factor (vWF) and fibronectin (Fn), as a by-product, were prepared from human plasma cryoprecipitate by a three-step chromatographic procedure. The respective final products were concentrated (80 U RCo/ml and 5 g/l, respectively), highly purified (specific activity >180 U RCo/mg protein and >95%, respectively) and biologically active. |
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| Keywords: | Adhesive proteins Chromatography Purification Von Willebrand factor |
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