| 蚯蚓纤溶酶对人肝癌细胞侵袭转移潜能的影响 |
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| 引用本文： | 陈洪,沈珊珊,张治国,陈宝安. 蚯蚓纤溶酶对人肝癌细胞侵袭转移潜能的影响[J]. 江苏医药, 2008, 34(4): 383-385 |
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| 作者姓名： | 陈洪 沈珊珊 张治国 陈宝安 |
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| 作者单位： | 东南大学附属中大医院,南京,210009;南京农业大学 |
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| 基金项目： | 南京市人事局留学归国人员科研择优资助项目 , 东南大学校科研和教改项目 |
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| 摘 要： | 目的 探讨蚯蚓纤溶酶(EFE)对人肝癌细胞系SMMC-7721细胞侵袭、转移潜能的影响.方法 将不同浓度EFE作用于体外培养的SMMC-7721细胞,通过细胞-基质粘附实验检测SMMC-7721细胞与基质胶(matrigel)黏附性,transwell小室模型测定细胞侵袭能力和迁移能力的改变,逆转录聚合酶链反应(RT-PCR)和蛋白印迹(Western blot)分别检测不同药物浓度作用后细胞内黏着斑激酶(FAK)mRNA及蛋白表达量的变化.结果 与对照组相比,EFE 2、4、6 uku/ml作用于SMMC-7721细胞能降低肝癌细胞与基质胶的黏附性(P<0.01)、降低SMMC-7721细胞的侵袭力及迁移能力(P<0.05或P<0.01);同时能够下调FAK mRNA和蛋白的表达(P<0.01).结论 EFE能抑制肝癌SMMC-7721细胞的侵袭和转移,其机制可能与其抑制FAK的表达有关,EFE具有潜在的抗肝癌细胞侵袭、转移作用.
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| 关 键 词： | 蚯蚓纤溶酶 肝癌 黏着斑激酶 |
| 文章编号： | 0253-3685(2008)04-0383-03 |
| 修稿时间： | 2007-01-17 |
Effect of earthworm fibrinolytic enzyme on the inhibition of invasion and metastasis in hepatocellular carcinoma cell |
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| CHEN Hong,SHEN Shanshan,ZHANG Zhiguo,et al.. Effect of earthworm fibrinolytic enzyme on the inhibition of invasion and metastasis in hepatocellular carcinoma cell[J]. Jiangsu Medical Journal, 2008, 34(4): 383-385 |
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| Authors: | CHEN Hong SHEN Shanshan ZHANG Zhiguo et al. |
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| Affiliation: | CHEN Hong,SHEN Shanshan,ZHANG Zhiguo,et al.Department of Gastroenterology,Alliliated Zhongda Hospital,Southeast University,Nanjing 210009 CHINA |
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| Abstract: | Objective To investigate the effect and possible mechanism of earthworm fibrinolylytic enzyme (EFE) on the inhibition of invasion and metastasis in hepatocellular carcinoma (HCC) cell line SMMC-7721. Methods The HCC cell line SMMC-7721 was cultured in vitro and treated with various concentrations of EFE.The inhibitory effect of EFE on the cell adhesion to extracellular matrix protein was investigated. Matrigel-transwell chamber method was used to evaluate the cell abilities of metastasis and chemotaxis.The ... |
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