肾病综合征患儿系膜增殖性肾炎人类白细胞抗原DR基因频率

利用寡核苷酸探针杂交技术及聚合酶链反应技术，检测了临床表现为肾病综合征的36例患儿人类白细胞抗原（HLA）－DR基因频率的分布；其中9例为IgA肾病，27例为非IgA系膜增殖性肾炎。分别与255例正常汉族人HLA－DR基因频率进行比较，发现IgA肾病患儿HLA－DR4基因频率明显高于正常人［DR4：19．4％vs9．0％，Pc＜0．05，相对危险系数（OR）=2．98］；而非IgA系膜增殖性肾炎HLA－DR12（5）基因频率明显高于正常人[DR12（5）：13．9％vs3．9％，Pc＜0．01，OR=4．52]。提示HLA－DR4、HLA－DR12（5）分别与临床表现为肾病综合征的儿童IgA肾病和非IgA系膜增殖性肾炎具有明显相关性。

Analysis of HLA-DR gene frequencies in primary nephrotic syndrome

To investigate the relationship between HLA-DR gene frequencies and primary nephrot-ic syndrome (NS) in children (age range: 2~14 years), we used polymerase chain reaction (PCR) anda set of 30 sequence-specific oligonucleotide (SSO) probes to determine the HLA-DR gene frequencies.Of the 36 children, nine were diagnosed as IgA nephropathy (IgAN) and the other 27 were non-IgAmesangial proliferative glomerulonephritis (MsPGN). DNA samples extracted from peripheral bloodlymphocytes digested with proteinase K were subjected to PCR for 30 cycles and then to dot-blot hy-bridization with 30 probes labelled with Y-32 P-ATP. We found that there was a higher gene frequencyof DR4 in IgAN children with NS as compared with normal control (19. 4%vs9. 0%, Pc= 0. 02,RR=2. 98), and a higher gene frequency of DR12 (5) in non-IgA MsPGN children with NS (13. 9 %vs3. 9 %,Pc= 0. 006, RR = 4. 52). In conclusion, the association of HLA-DR4 and DR12 (5) with primary NS inchildren suggested that some immunogenetic situation be responsible for the immunological mechanism inChinese children with primary NS.