| 同步多色荧光原位杂交技术的建立及临床应用 |
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| 引用本文： | 黎青,孙筱放,陈欣洁,孔舒,郑育红,黄艳仪. 同步多色荧光原位杂交技术的建立及临床应用[J]. 北京大学学报(医学版), 2005, 37(1): 45-47 |
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| 作者姓名： | 黎青 孙筱放 陈欣洁 孔舒 郑育红 黄艳仪 |
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| 作者单位： | 广州市第二人民医院妇产科研究所,广州,510150;广州市第二人民医院妇产科研究所,广州,510150;广州市第二人民医院妇产科研究所,广州,510150;广州市第二人民医院妇产科研究所,广州,510150;广州市第二人民医院妇产科研究所,广州,510150;广州市第二人民医院妇产科研究所,广州,510150 |
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| 摘 要： | 目的:建立同步多色荧光原位杂交技术在外周血、羊水及单个胚胎细胞及骨髓细胞等样本中的实验方法,探索其在细胞遗传学、产前诊断、胚胎种植前诊断以及在血液病中的应用. 方法: 取外周血淋巴细胞、羊水细胞和不适于胚胎移植及冷藏的受精胚胎细胞、骨髓细胞分别制片,用相应的荧光标记的探针进行原位杂交,荧光显微镜取图分析. 结果:建立获得稳定可行的各类标本的实验方法. 结论:应用多色荧光原位杂交技术可对染色体进行快速准确的诊断,并可应用于产前诊断和胚胎种植前诊断及血液病的诊断.
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| 关 键 词： | 原位杂交 荧光 产前诊断 植入前诊断 |
| 文章编号： | 167X1-167X(2005)01-0045-03 |
| 修稿时间： | 2004-11-11 |
The establishment and clinical application of multicolor fluorescence in situ hybridization |
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| Qing Li,Xiao-fang Sun,Xin-jie Chen,Shu Kong,Yu-hong Zheng,Yan-yi Huang. The establishment and clinical application of multicolor fluorescence in situ hybridization[J]. Journal of Peking University. Health sciences, 2005, 37(1): 45-47 |
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| Authors: | Qing Li Xiao-fang Sun Xin-jie Chen Shu Kong Yu-hong Zheng Yan-yi Huang |
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| Affiliation: | Guangzhou Second People's Hospital, Guangzhou Research Institute of Obstetrics and Gynecology, Guangzhou 510150, China. qinglicn@sina.com.cn |
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| Abstract: | Objective: To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples. Methods: FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene. Results: FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations. Conclusion: Multi-color FISH is clinically useful tool, which can be used as an adjunct to conventional chromosome analysis for prenatal diagnosis , preimplantation genetic diagnosis and diagnosis of leukemia. |
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| Keywords: | In situ hybridization fluorescence Prenatal diagnosis Preimplantation diagnosis |
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