补肾壮骨中药对大鼠去卵巢后早期白细胞介素6介导的骨髓源性破骨细胞形成的影响

目的 观察补肾壮骨中药对大鼠去卵巢后早期骨髓源性破骨细胞形成能力和骨髓细胞表达白细胞介素6(IL-6)、IL-6受体(IL-6R)、gp130基因的影响。方法 健康3月龄雌性SD大鼠72只，其中24只为假手术对照组；48只去卵巢，随机分为去卵巢组和中药组，每组24只。分别于去卵巢后2、4、6、12周每组各取6只大鼠骨髓细胞作细胞培养和提取RNA，培养第6天分别作瑞氏-吉姆萨染色和抗酒石酸酸性磷酸酶(TRAP)染色，骨髓细胞TRIZOL提取总RNA。结果 去卵巢后2周，去卵巢组破骨细胞形成数即多于对照组(P<0．05)，骨髓细胞IL-6和IL-6R基因表达均显著升高(P<0．05，P<0．01)，第4～6周。上述改变达高峰，并持续至第12周；从4～12周，上述各指标中药组均明显低于去卵巢组(P<0．05，P<0．01)。以上各组全程未见gp130基因表达水平有明显变化。结论 本方所用补肾壮骨中药可以抑制大鼠去卵巢后骨髓源性破骨细胞的生成，这一效应至少部分与其抑制骨髓细胞在去卵巢后过度表达IL-6、IL-6R有关。

Effects of Chinese Herbs for Supplementing Shen and Strengthening Bone on IL-6 Mediated Myelogenic Osteo clasts Formation of Ovariectomized Rats in Early Stage

To observe the effect of Chinese herbs for supplementing Shen and strengthening bone (HB) on myelogenic osteoclasts formation, and gene expression of interleukin-6 (IL-6), IL-6 receptor (IL-6R) and gp130 in bone marrow. Methods Seventy-two healthy female SD rats of 3 months, were randomly divided into three groups, 24 in the sham-operated group (A), 24 in the ovariectomized group (B) and 24 in the after o variectomy HB treated group (C). Bone marrow cells of 6 rats from each group were respectively collected and cultured at four time points (2nd, 4th, 6th and 12th weeks after operation) . After 6 days of culture, the bone marrow cells were differentiated by Wright-Giemsa stain and TRAP stain, and total RN A in them was extracted by TRIZOL. Results Beginning from the 2nd week, the osteoclasts formation in Group B was higher than that in Group A (P<0.05), and IL-6, IL-6R gene expression significantly increased in Group B (P<0.05 or F< 0 .01) . These changes reached the peak in the 4th to 6th week, with the level maintained to the 12th week. As for comparison of Group B and C, the above-mentioned changes were significantly weakened in the latter ( P < 0.05 or P < 0.01). No significant change of gp130 gene expression revealed in the whole course in either group. Conclusion HB could inhibit the myelogenic osteoclasts formation in ovariectomized rats, this effect may be correlated with, partially at least, its inhibitory effect on the over-expressed IL-6 and IL-6R gene expression in myelocytes after ovariectomy.