| 乙肝相关性肝癌患者中Foxp3启动子区甲基化及其表达的研究 |
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| 引用本文： | 党 珊 陈 谱 张冰斐等. 乙肝相关性肝癌患者中Foxp3启动子区甲基化及其表达的研究[J]. 陕西医学杂志, 2014, 0(2): 131-133 |
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| 作者姓名： | 党 珊 陈 谱 张冰斐等 |
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| 作者单位： | [1]陕西省人民医院,西安710068 [2] 西安交通大学医学院第一附属医院内分泌实验室,西安710068 |
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| 基金项目： | 陕西省科技计划项目（2012K13-01-14） |
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| 摘 要： | 目的:研究乙肝相关性肝癌患者中Foxp3启动子区甲基化及其表达,探讨Foxp3在乙肝相关性肝癌发病中的意义。方法:使用real time RT-PCR检测34例肝癌组织中Foxp3mRNA的水平,通过焦磷酸测序的方法检测Foxp3的甲基化水平。结果:HBV阳性肝癌组织中Foxp3mRNA表达水平高于HBV阴性组,具有显著差异;HBV阳性与阴性肝癌组织中Foxp3的甲基化水平不具有统计学差异。结论:原发性肝癌患者中HBV感染使Foxp3mRNA表达升高,Foxp3mRNA表达升高可能不是或不完全是通过甲基化修饰来调控的。
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| 关 键 词： | 肝肿瘤 乙型肝炎病毒 DNA甲基化 @Foxp3 |
The methylation level of Foxp3 promoter in HBV-related hepatocellular carcinoma |
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| Affiliation: | Shaanxi Provincial People＇s Hospital(Xi＇an 710068) Dang Shan Chen Pu Zhang Bingfei et al |
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| Abstract: | Objective ：To study the methylation level of Foxp3 promoter in HBV- related hepatocellular carcinoma ,to discuss the significance of Foxp3 in pathogenesis of HBV- related hepatocellular carcinoma .Methods ：The methylation status of Foxp3 was detected by using pyrosequencing method ,the expression levels of Foxp3 mR-NA in 34 hepatocellular cancer tissues were analyzed by real-time quantitative PCR .Results ：Significantly Higher ex-pression level was observed for Foxp3 mRNA in HBV- related HCC samples compared with HBV negative ones ;The methylation levels in HBV positive and negative HCC samples had no significant diffrence .Conclusion ：In HCC patients ,HBV infection increases Foxp3 mRNA level and Foxp3 mRNA expression may not be ,or not entirely regu-lated by methylation modification . |
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| Keywords: | Liver neoplasms Hepatitis B virus DNA Methylation |
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