Th22细胞及IL-22在特应性皮炎患者外周血中的表达及意义

目的研究不同严重程度特应性皮炎(AD)患者Th22细胞及白细胞介素(IL)-22的变化,探讨其在AD发病中的作用。方法采用流式细胞术检测不同严重程度AD患者外周血Th22细胞的比例,采用酶联免疫吸附试验(ELISA)测定外周血中IL-22表达水平,以健康成人作为对照。结果轻中度AD患者与正常人比较,外周血Th22细胞比例、IL-22水平增高(P0.05);重度AD患者与正常人比较,外周血Th22细胞比例及IL-22水平明显增高(P0.01);重度AD患者与轻度AD患者比较,外周血Th22细胞比例及IL-22水平增高(P0.05);重度AD患者与中度AD患者比较,外周血Th22细胞比例及IL-22水平增高(P0.05)。结论 Th22细胞及IL-22与AD的严重程度呈正相关。

Significance and Expression of Th22 and IL-22 in Perpheral Blood of the Patients with Atopic Dermatitis

Objective In order to investigate the variations of Th22 and IL-22 in peripheral blood of the patients with different severity atopic dermatitis (AD) and explore their significances.Methods The percentage of Th22 cells in peripheral blood of patients with AD was detected by flow cytometry,the levels of IL-22 were detected by enzyme-linked immuno sorbent assay (ELISA).Meanwhile,healthy adults were taken as controls.Results The percentage of Th22 cells and the level of IL-22 were increased in patients with mild and moderate AD compared with those in healthy controls (P＜0.05).The percentage of Th22 cells and the level of IL-22 were significantly higher in patients with severe AD than those in healthy controls (P＜0.01).The percentage of Th22 cells and the level of IL-22 was higher in patients with severe AD compared with those in patients with mild AD (P＜0.05).The percentage of Th22 cells and the level of IL-22 were higher in patients with severe AD compared with theose in patients with moderate AD (P＜0.05).Conclusion Th22 cells radio and IL-22 level are positively correlated with the severity of AD.