EB病毒microRNA Bart6-5p表达对鼻咽癌上皮间质转化的影响

 目的 探讨EB病毒microRNA Bart6-5p表达对鼻咽癌细胞上皮间质转化（epithelial mesenchymal transition，EMT）的影响。方法 采用实时荧光定量PCR检测EB病毒阳性的鼻咽癌细胞株C666-1和淋巴瘤细胞株Akata、Daudi、Raji及Namalwa中microRNA Bart6-5p和Dicer mRNA的表达，在恒定携带EB病毒且microRNA Bart6-5p表达最高的鼻咽癌细胞株C666-1中抑制microRNA Bart6-5p表达后检测Dicer和与鼻咽癌EMT相关分子标志物E-cadherin、Vimentin、ZEB1和ZEB2 mRNA的表达。结果 EB病毒阳性细胞株中microRNA Bart6-5p mRNA表达水平按C666-1、Akata、Daudi、Raji及Namalwa顺序依次递减（P＜0.05），而Dicer mRNA表达水平则依次递增（P＜0.05）。在恒定携带EB病毒且microRNA Bart6-5p表达最高鼻咽癌细胞株C666-1中抑制microRNA Bart6-5p表达后，Dicer mRNA表达呈升高趋势（P＜0.05），与鼻咽癌EMT相关分子标志物E-cadherin mRNA表达呈升高趋势（P＜0.05）， Vimentin、ZEB1及ZEB2 mRNA表达则呈下降趋势（P＜0.05）。结论 EB病毒microRNA Bart6-5p可能通过调控Dicer基因表达而进一步影响鼻咽癌的上皮间质转化过程。

Role of EBV microRNA Bart6-5p in the epithelial-mesenchymal transition in nasopharyngeal carcinoma

Objective To investigate the ability of Epstein-Barr virus（EBV） microRNA Bart 6-5p to regulate Dicer expression and thereby influence the epithelial-mesenchymal transition（EMT） in nasopharyngeal carcinoma cells. Methods Expression of microRNA Bart6-5p and Dicer were quantitated using real-time quantitative PCR in the EBV-positive nasopharyngeal carcinoma cell line C666-1 and lymphoma cell lines Akata，Daudi，Raji and Namalwa. In C666-1 cells with the highest EBV load，microRNA Bart6-5p expression was inhibited and subsequent changes in levels of EMT-related molecular markers were examined. Results Bart6-5p expression followed the trend C666-1>Akata>Daudi>Raji>Namalwa，while Dicer followed the inverse trend. Inhibiting Bart6-5p expression in C666-1 increased expression of Dicer and the positive EMT marker E-cadherin，while it decreased expression of the negative EMT markers vimentin，ZEB1 and ZEB2. Conclusion EBV microRNA Bart6-5p can influence the EMT in nasopharyngeal carcinoma by regulating Dicer expression.