Oct4蛋白在左右半结肠癌中表达的比较及预后分析

  目的  探讨人结肠癌组织中Oct4蛋白表达情况，并分析其预后价值。  方法  选取2008年1月至2011年12月于无锡市锡山人民医院行左半结肠癌根治术的标本89例，右半结肠癌根治术的标本77例，共166例。应用免疫组织化学检测全部结肠癌组织中Oct4蛋白的表达，并对病理参数及预后情况进行分析。  结果  免疫组织化学法染色结果显示Oct4蛋白在左半结肠癌组织中阳性表达率为68.54%，右半结肠癌组织中阳性表达率为71.43%，两者无显著差异（P>0.05）；Oct4表达水平在右半结肠癌组织中与组织学分级、淋巴结转移及Dukes分期呈正相关，而在左半结肠癌组织中仅与组织学分级及Dukes分期呈正相关，与淋巴结转移情况无显著相关；另外，左半结肠癌患者5年生存率明显高于右半结肠癌患者（P < 0.01），且Oct4表达与5年总体生存情况无显著相关；右半结肠癌患者中，Oct4过表达组5年总体生存明显低于阴性组（P < 0.05）。Cox生存分析提示仅右半结肠癌中高水平Oct4提示着预后不佳。  结论  Oct4在左、右半结肠癌进展中起到了不同的作用，为探索结肠癌诊疗新靶点提供理论依据。 

Expression of Oct4 and its prognostic role in left-and right-side colon cancer

Objective:To investigate the expression and prognostic value of the Oct4 protein in colon cancer.Methods:Immunohistochemical technique was used to examine the expression of Oct4 protein in 89 left side colon cancer(LCC)tissues and 77 right-side colon cancer(RCC) tissues.The relationship among Oct4 expression,clinicopathological parameters,and the prognostic value of Oct4 in colon cancer was analyzed.Results:The positive rate of Oct4 protein in LCC was 68.54%,and that in RCC was 71.43%.There was no significant difference between the two values.In addition,Oct4 expression in RCC was positively correlated with histological grade,lymph node metastasis,and Dukes staging.By contrast,Oct4 expression in LCC was only positively correlated with histological grade and Dukes staging.In survival analysis, the 5-year survival rate of LCC was significantly higher than that in RCC(P<0.01).In patients with LCC,no obvious correlation was found between positive and negative Oct4 expression levels in OS.In patients with RCC,Oct4 overexpression indicated poor prognosis(P<0.05). Also,in Cox survival analysis,Oct4 overexpression indicated poor prognosis in RCC but not in LCC.Conclusion:These results indicated that Oct4 plays different roles in LCC and RCC.These roles can be used as theoretical basis for exploring new targets for the diagnosis and treatment of colon cancer.