声门上型喉癌N0颈淋巴结微灶转移的免疫组化检测

提高声门上型喉癌颈癌转移淋巴结微小转移灶的检出率。方法应用抗细胞蛋白单克隆抗体，ＩＳＡＢ免疫组化法对４６例常规临床病理颈部淋巴结阴性的颈深上淋巴结进行监测。

Immunohistochemical evaluation of micrometastases to cervical lymph nodes in supraglottic cancer]

OBJECTIVE: Supraglottic carcinomas of larynx tends to metastasize to cervical lymph nodes. The regular pathological method of diagnosis is easy to have the micrometastasis missed, and serial-sectioning is too difficult be used in the regular pathological examinations. The aim of this study was to evaluate the value of immunohistochemical assay in the detection of micrometastasis. METHODS: Monoclonal antibodies against cytokeratin (AE1/AE3) were used to detect the cervical lymph nodes of 46 cases (50 sides of neck) of supraglottic carcinoma with pathologically negative findings (pN0). RESULTS: Micrometastases were identified in 13 lymph nodes of 11 cases. CONCLUSION: It is suggested that the detection of lymph node micrometastases with immunohistochemical method is more sensitive than that of regular pathologic method. It is comparable to serial sectioning. Therefore, It is a valuable clinical method to detect the early lymph node metastasis.