| 人工蛹虫草药材高效毛细管电泳指纹图谱研究 |
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| 引用本文： | 叶斌,宋丽艳,于荣敏. 人工蛹虫草药材高效毛细管电泳指纹图谱研究[J]. 中药材, 2007, 30(1): 28-30 |
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| 作者姓名： | 叶斌 宋丽艳 于荣敏 |
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| 作者单位： | 暨南大学药学院,广东广州,510632 |
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| 摘 要： | 目的:利用高效毛细管电泳(HPCE)技术建立人工蛹虫草药材的指纹图谱。方法:采用50 cm×75μm毛细管柱,以0.5 mmol/L硼砂溶液为缓冲液,运行电压为20 kv,检测波长为254 nm,柱温25℃。结果:建立的指纹图谱中共有11个共有峰,且方法学考察符合规定的标准。结论:该法准确简便,可作为控制人工蛹虫草中药材内在质量的有效手段。
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| 关 键 词： | 人工蛹虫草 高效毛细管电泳 指纹图谱 |
| 文章编号： | 1001-4454(2007)01-0028-03 |
| 修稿时间： | 2006-08-08 |
Study on HPEC Fingerprint of Cultured Cordyceps militaris |
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| YE Bin,SONG Li-yan,YU Rong-min. Study on HPEC Fingerprint of Cultured Cordyceps militaris[J]. Journal of Chinese medicinal materials, 2007, 30(1): 28-30 |
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| Authors: | YE Bin SONG Li-yan YU Rong-min |
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| Affiliation: | College of Pharmacy, Jinan University, Guangzhou 510632, China |
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| Abstract: | OBJECTIVE: To investigate the High Performance Capillary Electrophoresis (HPCE) fingerprint of cultured Cordyceps militaris (L.) Link. METHODS: Separation was performed on a 50 cm x 75 microm uncoated capillary with 0.5 mmol/L borate solution (pH 9. 18) as HPEC buffer. The run voltage was 20 Kv, temperature 25 degrees C and the DAD detection was set at 254nm. RESULTS: Fingerprint consisted of 11 common peaks. The validation of methods was satisfied with the requirements for SFDA's technical regulations. CONCLUSION: The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris (L.) Link. |
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| Keywords: | Cultured Cordyceps militaris(L.) Link HPCE Fingerprint |
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