Interference by D-mannitol on serum D-arabinitol determination by enzymatic assay |
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Authors: | Matsuda Y Ohta Y Tsukada Y Nishikiori H Sakai T Yuasa S Fujita N Yoshimura M |
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Institution: | Marukin Shoyu Co., Ltd., Kyoto. |
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Abstract: | We examined a disparity in D-arabinitol values between two commercial assay kits, LABOFIT and ARABINITEC-AUTO. The determined values by the former were increased by 26%(y = 0.2643x) because of concomitant D-mannitol, whereas those by our newly developed ARABINITEC-AUTO was increased only by 2%(y = 0.0242x). Of 109 samples, 5 samples were found to contain more than 100 mumol/l of D-mannitol. A clear relation(r = 0.89) was noted between the degree of disparity between measurements by the two methods and D-mannitol concentrations in samples. Thus, we have proved that the disparity is mainly caused by D-mannitol. |
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