切割海马伞海马中56 kD差异蛋白的质谱分析

目的:探讨切割海马伞海马中具有生物活性的56kD差异蛋白的组成成份及其功能。方法:切割SD大鼠海马伞后14d海马进行非变性聚丙烯酰胺凝胶电泳(NativePAGE),应用电喷雾质谱分析、蛋白质数据库和文献分析等方法对56kD差异蛋白进行检测和功能分析。结果:质谱分析找到了33组肽段,这些蛋白按照其功能可以分为:细胞骨架蛋白、参与代谢的酶、信号传递、蛋白降解、核酸水解、氧化应激、神经递质运输、突触形成、功能未知蛋白。结论:切割海马伞海马中56kD差异蛋白中含有功能涉及神经干细胞迁移、分化的蛋白质。

Mass spectrometry analysis of the 56 kD proteins in the fimbria-transected hippocampus

Objective:To explore the ingredients and functions of the active 56 kD proteins from the fimbria-transected hippocampus. Methods:The Sprague-Dawley rats'hippocampus on the 14th day after fimbria transection was used for Native-PAGE.Electrospray ionization mass spectrometry,protein data bank and bibliometrics were applied to identify the 56 kD proteins and analyze their functions.Results:Mass spectrometry analysis of 56 kD protein found 33 groups of peptides which were divided into several functional groups:cytoskeletal protein,enzyme in metabolism,signal transduction,protein degradation,nucleic acid hydrolysis,oxidative stress,neurotransmitter transport,synapse formation and unknown function protein. Conclusion:56 kD proteins in the fimbria-transected hippocampus include some proteins inducing migration and differentiation of neural stem cells.