静脉药瘾者丙型肝炎病毒感染的分子流行病学

Molecular epidemiology of HCV infection in intravenous drug abusers

AIM To investigate the prevalence of HCV infection in Intravenous drug users and molecular biological characteristics of HCV. METHODS The serum samples from 284 intravenous drug abusers with different levels of alanine aminortansferase ALT) were obtained, and anti-HCV lgG was detected by ELISA, HCV typed by RT-PCR and restriction fragment length polymorphism (RFLP) analysis of the 5 untranslated region. of the 284 subjects, 191 were men and 93 were women, aged 21-36 years. RESULTS Anti-HCV lgG was positive in 40.5% (115/284) and HCV-RNA in 34.5% (98/284) with intravenous drug abusers. The HCV 1b and HCV 2a ganotypes and the 1b/2a mixed ganotype accounted for 33.7%, 46.9% and 19.4% of HCV infection, respectively. The distribution of HCV ganotypes in the individuals with different levels of ALT was identical. CONCLUSION Infection of HCV 2a genotype was predominant in the intravenous drug users from Chongqlng followed by HCV lb and 1b/ 2a mixed ganotypes. The different ganotypes of HCV were not related to the change of serum ALT levels.