High yield tissue dispersing method for cell culture |
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| Authors: | Héctor Montes de Oca |
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| Affiliation: | (1) Departamento de Bioquímica e Farmacologia, Escola Paulista de Medicina, Sao Paulo, SP, Brasil;(2) Present address: Escola de Medicina Veterinária, Universidade Federal da Bahia, Ondina, Salvador, Bahia, Brasil |
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| Abstract: | Summary A reliable method for preparing primary cultures from different organs, using a mixture of trypsin and Versene as dispersing agent(s) is described. This method provides high cell yields as well as monolayers free of cellular debris and suitable for determining viral cytopathogenic effects. |
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| Keywords: | primary cultures trypsin chelating agents tissue dispersal |
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