成骨生长肽对大鼠颅盖骨成骨细胞样细胞增殖和分化的影响

目的研究成骨生长肽(OGP)在体外对成骨细胞样细胞增殖分化的影响。方法在体外培养的新生大鼠颅盖骨成骨细胞样细胞中加入不同浓度的OGP(10-11mol/L~10-7mol/L),用MTT法检测细胞增殖,酶联免疫法检测细胞内碱性磷酸酶(ALP)的表达,RT-PCR法检测核心结合因子1(Cbfa1)mRNA的表达。结果OGP对成骨细胞样细胞增殖有促进作用(P<0.05),最大效应浓度为10-9mol/L;能增加细胞内ALP活性(P<0.05),最大效应浓度为10-8mol/L;可促进Cbfa1mRNA的表达(P<0.05),其最佳促进表达浓度为10-8mol/L,Cbfa1mRNA与β-actinmRNA像素值比值为0.89±0.02。结论OGP可促进成骨细胞样细胞增殖,增强Cbfa1mRNA的表达水平。

Osteogenic Growth Peptide Regulates Proliferation and Differentiation of Calvarial Osteoblast-like Cells of Rat Bones

Objective To investigate the effect of osteogenic growth peptide(OGP) to the proliferation and differentiation of osteoblast-like cells in vitro. Methods Calvarial osteoblast-like cells of rat bones were cultured individually in medium contained various concentrations (10~ -11 mol/L-10~ -7 mol/L) of OGP; and the cell proliferation was checked by MTT method, alkaline phosphatase activity was detected by ELISA and level of Cbfa1 mRNA was amplified with RT-PCR. Results In vitro, the OGP put in culture medium resulted in an increase of cell proliferation(P<0.05), ALP activity(P<0.05) and level of mRNA for Cbfa1(P<0.05) respectively. All used OGP concentrations (10~ -11 mol/L-10~ -7 mol/L) were effective but the maximum efficiency was obtained with 10~ -8 mol/L OGP. Conclusion Our results demonstrate that OGP stimulates proliferation and sequential differentiation in vitro of calvarial osteoblast-like cells of rat bones.