即刻法用于酶联免疫吸附试验室内质控的探讨

目的探讨即刻法在酶联免疫吸附试验（ELISA）室内质量控制（IQC）中的局限性,建立一种适用于日常质控更加准确有效的操作方法。方法采用即刻法和控制变异系数（CV）的方法同时统计初始阶段的质控数据,并用Excel 2000对质控数据自动统计、分析并绘制出即时累积的L-J质控图,将此操作方法用于日常质控进行评价。结果即刻法中前3次的质控数据直接影响随后的结果,如前3个质控数据的CV〈5%,随后统计的结果会出现假失控,前3个质控数据的CV〉10%,随后的结果会出现假在控。同时结合即时累积的L-J质控图可以直观质控结果和质控图曲线。结论采用即刻法进行室内质控时,应用控制变异系数的方法可减少假失控和假在控的结果,同时结合即时累积的L-J质控图能更准确有效的进行评价,效果明显优于单用即刻法。

Discussion about instant technique used for the internal quality control of immunology laboratory

Objective To discuss the limitations of instant technique in the internal quality control of enzyme lineked immunosorbent assay(ELISA)in immunology laboratory in order to establish a more accurate and effective method used for daily quality control.Methods The primary quality control(QC)data were calculated statistically by instant method and controlling coefficient of variation(CV)method,applied Excel 2000 software to make statistics automatically,analyzed the data in quality control and drew a immediate-a...