淋巴细胞白血病细胞分化与基因重排研究

采用ＭｃＡｂｓ免疫酶和ＰＣＲ技术研究３５例淋巴细胞白血病细胞分化起源及ＩｇＨ和ＴＣＲγ、ζ基因重排。结果表明：２０例表达Ｂ细胞表面标记的病例中，１５例Ｂ－ＡＬＬ分别起源于Ｂ祖、前前Ｂ、前Ｂ和成熟Ｂ细胞，５例Ｂ－ＣＬＬ均起源于成熟Ｂ细胞；１７例检测到ＩｇＨ基因重排，８例伴ＴＣＲｒ基因重排，２例伴ＴＣＲξ基因失。８例Ｔ－ＡＬＬ分别起源于幼稚、普通和成熟胸腺细胞，均检测到ＴＣＲｒ基因重排和／或ＴＣＲξ基

Study on the Cellular Differentiation and Gene Rearrangements of Lymphocytic Leukemias

The cellular differentiated origin and the rearrangements of IgH and TCRr.TCRs genes within 35 cases lymphocytic leukimta were studied by irnmunoenzymatic method with McAbs and PCR technique. The results suggested that the 15 cases of B-ALL origined from progenitor B, pre-pre-B,pre-B and mature B cells , respectively and all 8 cases of H-CLL from mature B cells. Out of them. 17 cases showed IgH gene rearrangement, 8 cases had TCRr gene rearrangement and deletion of TCR?gene was detected in 2 cases. 8 cases T-ALL was origined from immature, common and mature thymus cells, respectively and all were demonstrated the rearrangements of TCR. gerie and/or rearrangement or deletion of TCRa gene. The double-rearrangement of IgH and TCRr genes were found in 3 cases of biphenotype ALL and 3/4 cases of AUL showed TCRrgene rearrangement. The relation among cellular differentiated origin and antigen receptor gene rearrangements and its clinical significance were also discussed.