胚胎隔或隔与蓝斑组织联合移植治疗老年性痴呆动物模型的形态学研究

MORPHOLOGIC STUDY OF THE ANIMAL MODEL OF SENILE DEMENTIA TREATED WITH FETAL SEPTAL OR COMBINED SEPTAL WITH LOCUS CERULEUS TISSUE

An unilateral transection of the fimbria-fornix of aged rat was made in order to establish an animal model of senile dementia. The fetal septal or mixed septal and locus ceruleus tissue suspension was implanted to bilateral hippocampi of the lesion aged rats. After 16 weeks, ChAT and TH immunocytochemistry were used to observe the survival,growth and connection with host hippocampus of the grafts. AChE histochemistry was applied to study the distribution of the cholinergic fibers in host hippocampus and to quantify the cholinergic fiber densities in CA1,CA3 and dentate gyrus. The results showed that both septal and combined grafts could survive,the graft septal mass was larger than the combined one.Inside the graft,the abundant ChAT positive neurons distributed more in the center or along the boundary with host hippocampus.A few TH positive neurons were dispersed.The quantitative study of AChE-positive fibers showed that the mean fiber densities of CA1,CA3 and dentate gyrus were all significantly decreased in lesion ipsilateral hippocampi compared to that in control rats. The septal grafts could mostly reinnervate the lesioned hippocampi in all three areas,but the combined grafts could only increase the fiber density in CA1, it had no effect on CA3 and dentate gyrus.The combined septal with locus ceruleus graft did not act as well as the septal graft.