放射事故受照者血型糖蛋白A突变分析

目的研究血型糖蛋白Ａ（ＧＰＡ，ｇｌｙｃｏｐｈｏｒｉｎＡｌｏｃｕｓ）突变分析用作新的辐射生物剂量计。方法结合流式细胞分析和单克隆抗体标记技术，检测红细胞糖蛋白的变异。结果随访全国１０例放射源事故受照者，４人为ＭＮ杂合体，生物剂量在１４６～２９Ｇｙ，１人ＭＮ弱阳性。ＧＰＡＮＯ变异率与剂量线性回归曲线有显著意义，曲线斜率４１５３×１０－６／Ｇｙ，而ＮＮ没有显著意义。结论ＧＰＡ变异率剂量效应曲线二次线性拟合比一次线性好。二次线性重建远期辐射剂量高于一次模型，用原爆、切尔诺贝利、戈亚尼亚事故参数回顾剂量多偏高。

Assay of glycophorin A locus mutation in radiation accident victims

Objective Application of glycophorin A locus mutation assay as a new biodosimeter. Methods Using flow cytometry and immunolabeling to detect erythrocytes with variant glycoproteins on the surface membrane. Results Among 10 victims of radiation accidents 4 persons were determined as having MN heterozygotes with estimated dose of 14629 Gy, and one suspected of having MN. Hemizygote NO variant frequency showed significant doseresponse linear regression with slope of 415310-6/Gy, while NN showed no significant regression. Conclusion Doseresponse data are more fitted to the linearquadratic model than to the linear model. Dose reconstructed with the linearquadratic model is higher than that estimated with the latter. And doses estimated with standard curve of Hiroshima and Chernoby1 are higher than those assessed by assay of dicentrics plus rings after the accidents