骨髓增生异常综合征Cyr61和血管内皮细胞生长因子的表达及其相关性研究

目的 研究富半胱氨酸61(Cyt61)基因在骨髓增生异常综合征(MDS)不同亚型中的表达情况,探讨Cyr61基因在MDS发生、发展及向急性髓系白血病(AML)演变过程中的意义及其与血管内皮生长因子(VEGF)基因的关系.方法 采用RT-PCR和免疫组化SP法对28例MDS患者、12例AML(其中包括4例由MDS转变而来)患者和10例对照者骨髓单个核细胞(BMMNC)中Cyr61和VEGF mRNA和蛋白质水平进行检测.结果 Cyr61和VEGF mRNA在MDS组和AML组中的表达高于对照组,差异有统计学意义.Cyr61和VEGF mRNA在高危型MDS(RAEB-Ⅰ、RAEB-Ⅱ)(0.3998±0.2467,0.4775±0.1342)和AML患者中的表达(0.4594±0.1737,0.4967±0.2324)明显高于低危型MDS(RA、RARS、RCMD)患者中的表达(0.2213±0.1465,0.2872±0.2341)(P值均＜0.05).高危型MDS患者Cyt61和VEGF mRNA的表达水平和AML患者相比差异无统计学意义.MDS患者Cyr61和VEGF蛋白的表达水平高于对照组,差异有统计学意义.高危型MDS患者Cyr61和VEGF蛋白的表达水平[(38.7±2.9)%,(43.2±2.7)%]明显高于低危型MDS患者[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).MDS患者Cyr61和VEGF的表达呈明显正相关关系(r=0.8762,P＜0.01).结论 Cyr61和VEGF可能在MDS骨髓血管生成及病情发展过程中发挥重要作用.

Expression of cysteine rich 61 and vascular endothelial growth factor genes in patients with myelodysplastic syndromes and their relationship

Objective To explore the expression of Cysleine-rieh 61 (Cyr61) gene in the different subtypes of myelodysplastic syndromes (MDS),and the significance of Cyr61 in the genesis progression,and transformation of MDS and the relationship between Cyr61 and vascular endothelial grown factor(VEGF).Methods Reverse transeriptase-polymerase chain reaction (RT-PCR) and immunohistochemical S-P were used to detect mRNA and protein expressions of Cyr61 and VEGF in bone marrow mononuelear cells (BMMNC) from 28 MDS,12 acute myeloid leukemia(AML) patients,and 10 normal volunteers.Results Expressions of Cyr61 and VEGF were higher in MDS and AML patients than in controls(P ＜ 0.05).The expressions of Cyr61 and VEGF were significantly higher in high risk group (0.3998± 0.2647,0.4775 ±0.1342)than that in low risk M DS group(0.2213 ± 0.1465,0.2872±0.2341) (P ＜ 0.05),but no significant difference between high risk MDS and AML patients.Expressions of Cyr61 and VEGF protein were higher in MDS patients than in normal controls(P ＜0.05),and were significantly higher in high risk MDS group [(38.7±2.9)%,(43.2±2.7)%]than in low risk group[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).Expressions of Cyr61 and VEGF were significantly correlated(r =0.8762,P ＜0.01).Conclusion Cyr61 and VEGF may play a role in the angiogenesis and pathogenesis of MDS.