中国东北地区汉族群体6个miniSTR基因座多态性

目的:对173名东北汉族个体6个miniSTR基因座D10S1248,D14S1434,D22S1045,D4S2364,D2S441 and D1S1677的遗传多态性进行分析,获得群体遗传数据.方法:应用荧光标记多重PCR扩增,6个基因座在两个反应体系中进行.310遗传分析仪进行毛细管电泳,分析等位基因片段长度大小,应用数据收集和分析软件收集数据并进而分型.所得数据进行统计学分析.结果:6个miniSTR基因座在东北地区汉族群体中表现出很好的遗传多态性.6个基因座观察到的等位基因大小在67～115 bp,观察杂合度在0.728～0.827.6个基因座在中国东北地区汉族群体中的累积个体识别率为0.999 99,累计非父排除率为0.993 31.结论:6个基因座在东北汉族群体中均具有高度的遗传多态性,可以作为CODIS(联合DNA检索系统)基因座的有效补充,在群体遗传学及器官移植中有一定的应用价值.

Polymorphism study of six miniSTR loci in Han population from Northeast China

OBJECTIVE:Allele frequencies and forensic parameters for six miniSTR loci(D10S1248,D14S1434,D22S1045,D4S2364,D2S441 and D1S1677)were analyzed in a population of 173 unrelated Chinese Han population individuals from Northeast China.METHODS:The six miniSTR loci were preformed in two multiplex fluorescent PCR systems.ABI 310 Genetic Analyzer was utilized in capillary electrophoresis and the lengths of allele fragments were analyzed.Genetic data collection and analysis software were used for data collection and genotyping.Statistical analysis was performed to the data.RESULTS:The six miniSTR loci showed a moderate degree of polymorphism in Han population from Northeast China.The observed allele sizes were from 67 bp to 115 bp,and the observed heterozygosity ranged from 0.728 to 0.827.The combined power of discrimination and the combined power of exclusion for the six miniSTR loci in Northeast China were 0.999 99,and 0.993 31,respectively.CONCLUSION:The six miniSTR showed a moderate genetic polymorphism in Han population from northeast of China.Due to their small size of PCR amplicon,the six miniSTR could be useful supplements to the CODIS STRs,and they would be useful in population genetics and forensic analysis.