淋巴细胞合成生长激素对自身细胞增殖及γ－干扰素产生的影响

淋巴细胞能够合成和分泌生长激素已被证实。本文的目的在于观察免疫反应性生长激素（ｉｒＧＨ）在体外诱导淋巴细胞应答反应中发挥的作用。以特异性抗体和ｉｒＧＨ结合进行阻断试验，结果显示ＰＨＡ诱导的淋巴细胞增殖程度显著下降。４５μｇ／ｍｌ的免抗人ＧＨ抗体使淋巴细胞增殖的抑制率达４１．２％；使γ－干扰素（ＩＦＮ－γ）的产生从５６．３±２７．４ｎｇ／ｍｌ下降到４９．１±２３．０ｎｇ／ｍｌ（Ｐ＜０．０５）由此说明ｉｒＧＨ参与体外淋巴细胞应答活动的调节。

Effects of Lymphocyte-derived Growth Hormone (GH) on the Lymphoproliferation and IFN-gamma Production

It has been shown that lymphocytes produce immunoreactive GH (irGH). The activity of irGH in lymphocyte proliferation and IFN-gamma production was investigated with blocking test by anti-GH antibody in this study. The results showed that rat anti-human GH antibody (RAHGH) potently inhibited PHA-induced lymphoproliferation in serum free medium with a dose-dependent manner(r=0.984).Cells with RAHGH (45μgm/ml) had less (41.2%) incorporation of 3H-TdR in PHA-stimulated lymphocyte culture. Nonspecific antibody (RAMIg 50μg/ml) had no such effect. Neither RAHGH nor RAMIg affected the viability of cultured lymphocytes.In addition, treatment of RAHGH significantly decreased the level of Con-A induced IFN-gamma (from 56.3±27.4μg/ml down to 49.±23.0μg/ml,P<0.05), Collectively, these data indicated that lymphocyte-derived GH may play a critical role in replication and cytokine production of lymphocytes by autocrine/paracrine way.