Effects of glucagon-like peptide-1 (7-36)amide on insulin stimulated rat skeletal muscle glucose transport |
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Authors: | B. F. Hansen P. Jensen E. Nepper-Christensen B. Skjølstrup |
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Affiliation: | (1) Diabetes Biology, Novo Nordisk, Building 6B1.56, Novo Alle, DK-2880 Bagsvaerd, Denmark, DK |
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Abstract: | Glucagon-like peptide-1 binding sites have been reported in peripheral tissues including muscle. However, the potential extra-pancreatic effects of glucagon-like peptide-1(7-36)amide are controversial. To evaluate whether glucagon-like peptide-1(7-36)amide has any effects on skeletal muscle glucose transport, isolated rat soleus muscles were incubated in increasing concentrations of insulin (0–150 nmol/l) in the presence or absence of 1 nmol/l glucagon-like peptide-1(7-36)amide for 3 h. Subsequently glucose transport was measured as uptake of [3H]-O-methylglucose. It was found that glucagon-like peptide-(7-36)amide has a small but significant stimulating effect on skeletal muscle glucose transport independent of the insulin concentration (P<0.01). However, because of the magnitude of the observed effect, the physiological importance of glucagon-like peptide-1 (7-36)amide on skeletal muscle glucose metabolism is questionable. Received: 27 February 1998 / Accepted in revised form: 30 April 1998 |
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Keywords: | GLP-1(7-36)amide Glucose transport Skeletal muscle Rat Extrapancreatic effects |
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