皮肤归巢T淋巴细胞的趋化性及趋化因子受体的表达

目的 :探讨由接触性过敏原或结核菌素诱发的细胞介导的皮肤炎症反应中 ,皮肤归巢T淋巴细胞的趋化性及其趋化因子受体的表达。方法 :取斑贴试验阳性或结核菌素皮肤反应试验阳性受试者的皮肤标本 ,利用 4 8孔微趋化板技术、流式细胞仪等检测皮肤归巢T淋巴细胞的趋化性及其趋化因子受体的表达。结果 :CC型趋化因子eotaxin、MIP 1α和CXC型趋化因子IL 8对于皮肤归巢T淋巴细胞有趋化效应 ,但这些细胞对RANTES无反应。皮肤归巢T淋巴细胞上CCR3、CCR5、CXCR1和CXCR2呈动态表达 ,而粘附分子ICAM 1则是高表达。在体外培养基中 ,趋化因子受体的表达即趋化运动的能力和粘附分子的表达受IL 2和IL 4的调节。结论 :在细胞介导的皮肤炎症反应中 ,趋化因子及其受体通过与T细胞源性的生长因子IL 2和IL 4的相互作用决定了皮肤归巢T淋巴细胞的定向迁移、粘附、聚集和循环 ,同时也决定了嗜酸性粒细胞和嗜碱性粒细胞的聚集

Chemotaxis and Chemokine Receptor Expression of Skin-Homing T Lymphocytes

Objective: To investigate the chemotactic properties and chemokine receptor expression of skin homing T lymphocytes from a cutaneous cell mediated reaction initiated by a contact allergen or by tuberculin. Methods: The chemotactic activity of skin homing T lymphocytes towards chemokines and the expression of chemokine receptors on skin homing T lymphocytes from subjects were investigated with a positive patch test or a positive tuberculin skin test through a 48 well micro chamber technique and a flow cytometer. Results: The CC chemokines eotaxin,MIP 1 α and the CXC chemokine IL 8 were chemotactic for skin homing T lymphocytes, whereas they did not respond to RANTES. The CCR3, CCR5, CXCR1 and CXCR2 were dynamically expressed, whereas the adhesion molecule ICAM 1 was highly expressed on skin homing T lymphocytes. The expression of chemokine receptors, the chemotactic capacities, and adhesion molecule expression was modulated by withdrawal of IL 2 and IL 4 from the culture medium. Conclusion: Through the interaction with T cell derived growth factors IL 2 and IL 4, chemokines and chemokine receptors can determine the directional migration, adhesion, accumulation and recruitment of skin homing T lymphocytes and the accumulation of eosinophils and basophils during cell mediated immmune reactions in skin.