| bcl-2/bcl-xL反义寡核苷酸对乳腺癌细胞增殖和凋亡的影响 |
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| 引用本文： | 章骏,杨建辉. bcl-2/bcl-xL反义寡核苷酸对乳腺癌细胞增殖和凋亡的影响[J]. 实用癌症杂志, 2003, 18(5): 457-459 |
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| 作者姓名： | 章骏 杨建辉 |
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| 作者单位： | 200090,上海市市东医院肿瘤外科 |
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| 摘 要： | 目的 观察靶向bcl 2 /bcl xL基因的反义寡核苷酸 (ASODN )对乳腺癌细胞株MCF 7增殖和凋亡的影响。方法 将bcl 2 /bcl xLASODN及其对照序列通过脂质体转染MCF 7细胞。采用MTT法测定MCF 7细胞增殖 ,荧光显微镜定量检测MCF 7细胞凋亡率。结果 与对照序列相比 ,bcl 2 /bcl xLASODN能抑制MCF 7增殖和诱导其凋亡 (P <0 .0 1)。结论 bcl 2 /bcl xLASODN在乳腺癌治疗方面作为 1种新的化合物值得进一步研究
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| 关 键 词： | bcl-2 bcl-x L反义寡核苷酸 乳腺癌 细胞增殖 细胞凋亡 |
| 文章编号： | 1001-5930(2003)05-0457-03 |
The Effects of bcl-2/bcl-xL Antisense Oligodeoxyneucleotides on Proliferation and Apoptosis of Breast Cancer Cell Line MCF-7 |
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| ZHANG Jun,YANG Jian-hui. The Effects of bcl-2/bcl-xL Antisense Oligodeoxyneucleotides on Proliferation and Apoptosis of Breast Cancer Cell Line MCF-7[J]. The Practical Journal of Cancer, 2003, 18(5): 457-459 |
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| Authors: | ZHANG Jun YANG Jian-hui |
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| Affiliation: | ZHANG Jun,YANG Jian-hui. Department of Surgical Oncology,Shidong Hospital,Shanghai,200090 |
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| Abstract: | Objective To observe the effect of antisense oligodexynucelotides(ASODN) targeting bcl-2/bcl-xL on cell proliferation and apoptosis of breast cancer cell line MCF-7.Methods bcl-2/bcl-xL ASODN and control sequence were transfected into MCF-7 with lipofectin.The MCF-7 cell growth was assessed by MTT and cell apoptosis was detected by quantitative fluorescence microscope.Results Compared with control sequence,bcl-2/bcl-xL ASODN could inhibit the growth of MCF-7 and induce apoptosis(P<0.01).Conclusion The bcl-2/bcl-xL-antisense oligonucleotide has a value to further study as a novel compound for breast cancer therapy. |
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| Keywords: | Breast cancer bcl-2 gene bcl-xL gene Antisense oligodeoxyneucleotides(ASODN) |
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