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电离辐射对人外周血线粒体编码基因mRNA表达的影响
引用本文:张忠新,刘建功,张淑贤,段志凯. 电离辐射对人外周血线粒体编码基因mRNA表达的影响[J]. 癌变.畸变.突变, 2013, 25(1): 22-25. DOI: 10.3969/j.issn.1004-616x.2013.01.005
作者姓名:张忠新  刘建功  张淑贤  段志凯
作者单位:( 中国辐射防护研究院,山西 太原 030006 )
摘    要:目的: 观察γ射线对人外周血线粒体编码基因mRNA表达水平的影响,探讨线粒体编码基因用于辐射生物剂量估算的可行性。方法:用不同剂量水平 (0~5 Gy)的60Co γ射线照射3名正常人离体外周血样本,照射6 h后,提取总RNA,应用实时荧光定量PCR技术检测ND1,ND6,细胞色素C氧化酶亚基 (cytochrome C oxidase subunit,COX)I、II、III,三磷酸腺苷酶6 (adenosine triphosphatase,ATPase6),三磷酸腺苷酶8(adenosine triphosphatase,ATPase8) mRNA表达水平的变化,采用Origin 7.5拟合照射剂量与基因表达水平的剂量-效应关系曲线。结果:γ射线照射人外周血后,线粒体编码基因COXI及 ATPase6的相对表达水平在0~5 Gy剂量范围内表达先增加,3 Gy剂量点之后显现下降趋势,对这两个基因在0~3 Gy剂量范围内分别进行拟合,得到COXI 剂量-效应关系方程为Y=0.625 9+0.256 1D (r2=0.916 3,P<0.01); ATPase6基因表达剂量-效应关系方程为Y=0.218 9+ 0.806 0 D (r2=0.912 6,P<0.01),其他5个基因的相对表达水平与照射剂量间不存在明显的剂量-效应关系 (P>0.05)。结论:γ射线对人外周血线粒体编码基因COXI 及 ATPase6的mRNA表达水平具有明显的影响,这两个基因的表达水平与γ射线的照射剂量存在线性关系,有望用于辐射生物剂量的估算。

关 键 词:电离辐射  线粒体  基因表达  外周血  
收稿时间:2012-05-15
修稿时间:2012-09-21

Effects of radiation on mitochondrial gene expression in human peripheral blood cells
ZHANG Zhong-xin , LIU Jian-gong , ZHANG Shu-xian , DUAN Zhi-kai. Effects of radiation on mitochondrial gene expression in human peripheral blood cells[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2013, 25(1): 22-25. DOI: 10.3969/j.issn.1004-616x.2013.01.005
Authors:ZHANG Zhong-xin    LIU Jian-gong    ZHANG Shu-xian    DUAN Zhi-kai
Affiliation:(China Institute for Radiation Protection, Taiyuan 030006, Shanxi, China)
Abstract:OBJECTIVE:To explore the effect of ionizing radiation on mitochondrial gene expression in human peripheral blood cells. METHODS:Human peripheral blood samples were exposed to 60Co γ-ray at different doses,and its RNA was isolated from the white cells. The relative real-time quantitative PCR by standard curve method was set up using β-actin as internal standards. With this method,ND1,ND6,COXI,COXII,COXIII,ATPase6, ATPase8 gene expression changes after ionizing radiation wererecorded,the relationship between mitochondrial gene expression and dose was analyzed by software of origin 7.5.RESULTS:The gene expression of COXI and ATPase6 after radiation of peripheral blood cells increased with the irradiation doses. From 0-3 Gy range,the relationship between the ratio of COXI mRNA/β-actin could be represented as:Y=0.625 9+0.256 1 D (r2=0.916 3,P<0.01),the ATPase6 mRNA/ β-actin was:Y=0.218 9+ 0.806 0 D (r2=0.912 6,P<0.01).The relationship between the ratio of other mRNA/β-actin was not statistically different (P>0.05). CONCLUSION:Differential expression of COXI,ATPase6 mRNA was induced by ionizing radiation,so COXI and ATPase6 could be promising radiation biological dosimeters.
Keywords:
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