5-Br-PADAP直接光度法测定血清锌

目的建立一种快速、简便、灵敏的分光光度法测定血清锌。方法在表面活性剂TritonX-100存在下，用2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)作显色剂，不去蛋白直接光度法测定血清锌。结果该方法显色络合物最大吸收波长为558nm，线性范围达51.0μmol/L，表观摩尔吸光数为1.05×105L*mol-1*cm-1。回收率为98.1%～103.2%，批内和批间变异系数(CV)分别为2.1%与3.6%，与原子吸收分光光度法比较相关良好，Y=1.02X-0.41，r=0.9862，P＞0.05。48名健康人血清锌含量为(9.5～24.3)μmol/L(±2s)。结论该法血清用量少，不必去蛋白，具有操作快速、简便、结果灵敏可靠等优点，适合临床应用。

The direct assay for zinc in serum with 5-Br-PADAP

Objective To establish a simple. sensitive and stable method of determination of zinc in serum by UV-spectrophotometry. Methods Determination of serum zinc with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol(5-Br-PADAP) in the presence of surfactant Triton X-100. Results The maximum absorption of the complex compound was at 558 nm, the linearity of test was up to 51 μmol/L. The molar absorption coefficient is 1.05×105L/mol-1*cm-1.The recoveries ranged from 98.1% to 103%. The within run and between run CV are 2.1% and 3.6%. Comparing this method (Y) with AAS(X), we obtained the regression equation.Y=1.02X-0.41,r=0.9862,P＞0.05. The reference interval (±2s) was (9.5～24.3)μmol/L (n=48). Conclusions This method is simple, sensitive, stable, rapid and accurate for clinical application.