节球藻毒素在小鼠体内分布的研究

目的 研究和探讨节球藻毒素在动物体内分布的靶器官及其在靶器官中的细胞水平定位。方法 用核素^125Ⅰ标记节球藻毒素,将^125Ⅰ节球藻毒素标记物注入小鼠体内,分别经核素示踪和放射性自显影技术研究协^125Ⅰ节球藻毒素在小鼠体内整体水平和细胞水平的分布。结果 核素示踪显示,静脉注射、腹腔注射和口服3种不同途径进入体内的^125Ⅰ节球藻毒素,主要分布在肾脏,其次为肝脏。放射性自显影研究表明,^125Ⅰ节球藻毒素在肾脏和肝脏内分别定位于肾皮质的肾细胞核内和肝细胞核内。结论 肾脏和肝脏是节球藻毒素的2个靶器官。

Study on the distribution of nodularin in tissues and cell level in mice

Objectives To study the tissue distribution of nodularin in mice and the cellular location of nodularin in the target organs. Methods The nodularin was labeled with radioactive isotope 125 I and then was given to mice via oral, intraperitoneal and intravenous administration. The distribution of nodularin in target organs and the cellular location of nodularin were studied by radioisotope and autoradiography techniques. Results The radioisotope study results showed that nodularin was mainly distributed in the kidney and liver in mice. Further autoradiography study indicated that nodularin was distributed in the renal cell nuclei and liver cell nuclei.Conclusion The kidney and liver are the two main target organs for nodularin in mice.