同供体肝细胞预先输入对异种胰岛移植物存活影响的实验研究

应用胶原酶消化分离大鼠胰岛细胞，以Ｆｉｃｏｌｌ密度梯度离心纯化，同时分离大鼠肝细胞。以链脲霉素制备小鼠糖尿病模型２４只。随机分为３组。实验组小鼠预先输入供体大鼠肝细胞，６在后再经门静脉植入同供体经培养的胰２岛细胞。对照１组为空白对照组，不予任何处置，对照２组单纯植入胰岛细胞。

Effect of donor hepatocyte preinfusion on the survival of islet xenografts

The rat islets were isolated by using collagenase digestive method, purified by Fi-coll density gradient centrifugation, and rat hepatocytes isolated simultaneously.Twenty-four cas-es of diabetic models, developed in mice with streptozotocin, were divided into 3 groups randomly.In experimental group, donor rat hepatocytes were infused first, followed by transplantation of cultured islets from same donor via portal vein on the 6th day.In Control group 1 no intervention was performed, and in control group 2, simple infusion of islets was done.The results showed group.Significant difference was noted between the two groups(P＜0.05).The decreased blood glucose level were maintained about a week in experimental group.This result reveals that hepa-tocytes can induce immunotolerance and prolong the survival of xenografts to some extent.