体外受精-胚胎移植中生长激素在子宫内膜异位症患者中的作用

［摘要］ 〖HTH〗目的〖HTSS〗〖KG*2〗探讨生长激素（growth hormone，GH）在子宫内膜异位症(endometriosis，EMs)患者中的意义及其对卵巢黄素化颗粒细胞凋亡的影响。 〖HTH〗方法〖HTSS〗〖KG*2〗选择进行体外受精-胚胎移植（in vitro fertilization and embryo transfer,IVF-ET）助孕的EMs不孕患者（EMs组）40例，单纯输卵管因素不孕患者（对照组）40例，比较2组实验室指标及妊娠结局；放射免疫分析法检测2组取卵当日血清、卵泡液GH水平，密度梯度法提取颗粒细胞体外培养贴壁后，免疫细胞化学检测2组患者Bcl-2、Bax蛋白表达水平；将贴壁后EMs患者颗粒细胞按干预方式分为空白组和GH组，免疫细胞化学检测Bcl-2、Bax蛋白的表达水平。 〖HTH〗结果〖HTSS〗〖KG*2〗EMs组血清、卵泡液GH水平和颗粒细胞Bcl-2表达水平低于对照组，EMs组Bax表达水平高于对照组，差异均有统计学意义（P＜0.05）。GH组较空白组Bcl-2表达水平升高，Bax表达水平下降，差异有统计学意义（P＜0.05）。 〖HTH〗结论〖HTSS〗〖KG*2〗EMs患者的低GH水平可能引起颗粒细胞凋亡增加，从而影响卵母细胞质量及胚胎的发育潜能。一定浓度的GH可能改善EMs患者颗粒细胞凋亡状态。

Effect of growth hormone on patients with endometriosis in vitro fertilization and embryo transfer#br#

［Abstract］Objective〖HTSS〗To investigate the significance of growth hormone(GH) in patients with endometriosis(EMs) and its effect on apoptosis of ovarian luteinized granulosa cells. 〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of forty infertility patients with endometriosis who underwent in vitro fertilization-embryo transfer(IVF-ET) (EMs group) and forty infertility patients with simple fallopian tube factors who underwent IVF-ET(control group) were screened and enrolled in the study. The laboratory indicators and pregnancy outcomes of the two groups were compared. The serum GH levels and follicular fluid GH levels of the two groups on the day of oocyte retrieval were measured by radioimmunoassay. The expression of Bcl-2 protein and Bax protein in granulosa cells extracted by density gradient method were detected by immunocytochemistry after cell adherence in vitro culture. The granulosa cells of EMs patients were divided into blank group and GH group according to the intervention method after adherence.The expression levels of Bcl-2 protein and Bax protein were detected by immunocytochemistry. 〖HTH〗〖WTHZ〗Results〖HTSS〗The GH levels of serum and follicular fluid in EMs group were significantly lower than those in control group. The expression of Bcl-2 protein in granulosa cells of the EMs group was significantly lower than that of the control group .The expression level of Bax protein was higher than that of the control group, with statistically significant differences(P＜0.05). The expression of Bcl-2 protein increased in GH group compared with blank group, and the expression of Bax protein decreased in GH group compared with blank group. These differences were statistically significant(P＜0.05). 〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Low growth hormone levels in patients with endometriosis may cause an increase in granulosa cell apoptosis, which affects oocyte quality and embryonic developmental potential. A certain concentration of growth hormone may improve the granulosa cell apoptosis state in patients with endometriosis.