| 胰酶处理牛乳中黄嘌呤氧化酶的提纯及其应用研究 |
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| 引用本文： | 方允中,刘智峰,曹宜生. 胰酶处理牛乳中黄嘌呤氧化酶的提纯及其应用研究[J]. 军事医学科学院院刊, 1988, 0(5) |
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| 作者姓名： | 方允中 刘智峰 曹宜生 |
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| 作者单位： | 军事医学科学院放射医学研究所，军事医学科学院放射医学研究所，广州军区军事医学研究所 |
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| 摘 要： | 用Massey法从胰酶处理牛乳中提纯黄嘌呤氧化酶,但对其程续进行某些改进.用改进程续纯化的黄嘌呤氧化酶的比活性为3.95IU/mg蛋白质而Massey报道的原始程续纯化的酶比活性为3.5IU/mg蛋白质.前者高于后者.纯化的黄嘌呤氧化酶已成功地应用于生物化学与自由基生物学和医学的研究.
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| 关 键 词： | 胰酶处理牛乳 黄嘌呤氧化酶 |
STUDIES ON THE PURIFICATION OF XANTHINE OXIDASE FROM PANCREATIN-TREATED MILK AND ITS APPLICATION |
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| Fang Yunzhong,Liu Zhifeng,Cao Yisheng Institute of Radiation Medicine,Academy of Military Medical Sciences. STUDIES ON THE PURIFICATION OF XANTHINE OXIDASE FROM PANCREATIN-TREATED MILK AND ITS APPLICATION[J]. Bulletin of the Academy of Military Medical Sciences, 1988, 0(5) |
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| Authors: | Fang Yunzhong Liu Zhifeng Cao Yisheng Institute of Radiation Medicine Academy of Military Medical Sciences |
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| Abstract: | Massey's method was modified for the purification of xanthine oxidase from pancreatin-treated milk.The specific activity of xanthine oxidase purified by modified procedure being 3.95 IU/mg protein was higher than that by the original approach (3.5 IU/mg protein as reported by Massey).The purified enzyme had been successfully used in biochemistry,free radical biology and medicine. |
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| Keywords: | pancreatin-treated milk xanthine oxidase |
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